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Trans. R. Soc. B"],"published-print":{"date-parts":[[2023,6,19]]},"abstract":"<jats:p>\n                    We illustrate use of induced pluripotent stem cells (iPSCs) as platforms for investigating cardiomyocyte phenotypes in a human family pedigree exemplified by novel heterozygous RYR2-A1855D and SCN10A-Q1362H variants occurring alone and in combination. The proband, a four-month-old boy, presented with polymorphic ventricular tachycardia. Genetic tests revealed double novel heterozygous RYR2-A1855D and SCN10A-Q1362H variants inherited from his father (F) and mother (M), respectively. His father showed ventricular premature beats; his mother was asymptomatic. Molecular biological characterizations demonstrated greater\n                    <jats:italic>TNNT2<\/jats:italic>\n                    messenger RNA (mRNA) expression in the iPSCs-induced cardiomyocytes (iPS-CMs) than in the iPSCs. Cardiac troponin Ts became progressively organized but cytoplasmic RYR2 and SCN10A aggregations occurred in the iPS-CMs. Proband-specific iPS-CMs showed decreased\n                    <jats:italic>RYR2<\/jats:italic>\n                    and\n                    <jats:italic>SCN10A<\/jats:italic>\n                    mRNA expression. The RYR2-A1855D variant resulted in premature spontaneous sarcoplasmic reticular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients, Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations and increased action potential durations. SCN10A-Q1362H did not confer any specific phenotype. However, the combined heterozygous RYR2-A1855D and SCN10A-Q1362H variants in the proband iPS-CMs resulted in accentuated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis disorders, action potential prolongation and susceptibility to early afterdepolarizations at high stimulus frequencies. These findings attribute the clinical phenotype in the proband to effects of the heterozygous\n                    <jats:italic>RYR2<\/jats:italic>\n                    variant exacerbated by heterozygous\n                    <jats:italic>SCN10A<\/jats:italic>\n                    modification.\n                  <\/jats:p>\n                  <jats:p>This article is part of the theme issue \u2018The heartbeat: its molecular basis and physiological mechanisms\u2019.<\/jats:p>","DOI":"10.1098\/rstb.2022.0175","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T02:39:17Z","timestamp":1682908757000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Patient-specific induced pluripotent stem cell properties implicate Ca\n                    <sup>2+<\/sup>\n                    -homeostasis in clinical arrhythmia associated with combined heterozygous\n                    <i>RYR2<\/i>\n                    and\n                    <i>SCN10A<\/i>\n                    variants"],"prefix":"10.1098","volume":"378","author":[{"given":"Yafei","family":"Zhou","sequence":"first","affiliation":[{"name":"National Regional Children\u2019s Medical Center (Northwest); Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province; Xi\u2019an Key Laboratory of Children\u2019s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi\u2019an Children\u2019s Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University, No. 69, Xijuyuan Lane, Xi'an 710003, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Huang","sequence":"additional","affiliation":[{"name":"National Regional Children\u2019s Medical Center (Northwest); Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province; Xi\u2019an Key Laboratory of Children\u2019s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi\u2019an Children\u2019s Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University, No. 69, Xijuyuan Lane, Xi'an 710003, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leiying","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Electrophysiology of the Ministry of Education, and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Longmatan District, Luzhou 646000, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anmao","family":"Li","sequence":"additional","affiliation":[{"name":"National Regional Children\u2019s Medical Center (Northwest); Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province; Xi\u2019an Key Laboratory of Children\u2019s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi\u2019an Children\u2019s Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University, No. 69, Xijuyuan Lane, Xi'an 710003, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congshan","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Regional Children\u2019s Medical Center (Northwest); Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province; Xi\u2019an Key Laboratory of Children\u2019s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi\u2019an Children\u2019s Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University, No. 69, Xijuyuan Lane, Xi'an 710003, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Regional Children\u2019s Medical Center (Northwest); Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province; Xi\u2019an Key Laboratory of 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