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Trans. R. Soc. B"],"published-print":{"date-parts":[[2025,11,6]]},"abstract":"<jats:p>\n                    Cytomegalovirus (CMV) establishes lifelong latency and is linked to immunosenescence in older and immunocompromised individuals. We hypothesize that CMV drives systemic and tissue-specific immune changes that may contribute to cardiovascular disease (CVD). Thoracic aorta, blood and perivascular mediastinal adipose tissue from cardiac surgery patients (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 11) were processed within 30\u201360 min of excision. CMV IgG titres were quantified through ELISA to determine CMV status: CMV(\u2212) (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 4) and CMV(+) (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 7). Immune profiling was performed using flow cytometry and single-cell RNA sequencing. Analyses included MiloR and differential gene expression. Participants (mean age 69.7 \u00b1 8.4 years) were 80% male and 70% Caucasian. CMV(\u2212) and CMV(+) participants had mean IgG titres of 0.038 and 13.55 IU ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. CD8\n                    <jats:sup>+<\/jats:sup>\n                    T-cells expressing CD57\n                    <jats:sup>+<\/jats:sup>\n                    , GPR56\n                    <jats:sup>+<\/jats:sup>\n                    and CX3CR1\n                    <jats:sup>+<\/jats:sup>\n                    (CGC) were increased in the blood of CMV(+) participants. In the aorta of CMV(+) participants, CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells had decreased HLA-C expression and suppressed interferon-\u03b1 pathways. In contrast, the TNF-\u03b1 signalling pathway was increased. CMV infection shapes immune responses and in this pilot, we observed suppression of interferon-\u03b1 signalling and increased TNF-\u03b1-associated pathways in the aorta. Larger studies are needed to define how CMV-driven immune remodelling contributes to CVD.\n                  <\/jats:p>\n                  <jats:p>This article is part of the discussion meeting issue \u2018The indirect effects of cytomegalovirus infection: mechanisms and consequences.\u2019<\/jats:p>","DOI":"10.1098\/rstb.2024.0401","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T08:08:23Z","timestamp":1762416503000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Mapping CMV-related immune signatures in blood, aorta and perivascular mediastinal adipose tissue"],"prefix":"10.1098","volume":"380","author":[{"given":"Cassia","family":"Labeb","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN 37232, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laventa 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