{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:58:36Z","timestamp":1791388716840,"version":"4.3.3"},"reference-count":29,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p>The enterovirus 2B protein contains a putative amphipathic \u03b1-helix that includes three positively charged and one negatively charged residue. Previously, we observed that replacement of the glutamic acid-40 residue with a lysine residue (mutation 2B-E[40]K) in the amphipathic \u03b1-helix of the coxsackie B3 virus 2B protein resulted in a quasi-infectious phenotype. On one occasion, however, transfection of 2B-E[40]K RNA transcripts gave rise to a virus stock in which the mutation was retained. This study was aimed at elucidating the molecular mechanism underlying this observation. Sequence analysis of the viral RNA provided no evidence for a second-site suppression mutation that rescued the defect of the 2B-E[40]K mutation in <jats:italic>cis<\/jats:italic>. Therefore, the possibility was considered that the defect caused by the 2B-E[40]K mutation was complemented in <jats:italic>trans<\/jats:italic> by viable revertants that had emerged in the virus population. The transfection-derived virus stock indeed contained a small fraction of (pseudo)revertant viruses, carrying the original glutamic acid-40, threonine-40 or asparagine-40, rather than the introduced lysine-40. Consistent with the idea that the 2B-E[40]K virus is unable to grow without the aid of <jats:italic>trans<\/jats:italic>-acting wild-type(-like) proteins, only the (pseudo)revertant viruses were able to produce individual plaques. Further support for the idea of <jats:italic>trans<\/jats:italic>-rescue was obtained using a genetic complementation assay, which revealed the occurrence of a low level of <jats:italic>trans<\/jats:italic>-complementation of the 2B-E[40]K mutation by wild-type virus. This is the first report that provides evidence that a genetic defect in the enterovirus 2B protein can be complemented in <jats:italic>trans<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/0022-1317-83-2-341","type":"journal-article","created":{"date-parts":[[2015,8,26]],"date-time":"2015-08-26T16:24:17Z","timestamp":1440606257000},"page":"341-350","source":"Crossref","is-referenced-by-count":6,"title":["Trans-complementation of a genetic defect in the coxsackie B3 virus 2B protein"],"prefix":"10.1099","volume":"83","author":[{"given":"Frank J. 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