{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:34:46Z","timestamp":1789648486042,"version":"4.0.0"},"reference-count":26,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,8,1]]},"abstract":"<jats:p>Paralytic poliomyelitis results from destruction of motor neurons owing to poliovirus (PV) replication. Using a mouse model, we have previously shown that PV kills neurons of the central nervous system (CNS) as a result of apoptosis (Girard <jats:italic>et al<\/jats:italic>., <jats:italic>Journal of Virology<\/jats:italic> 73, 6066\u20136072, 1999). We report the development of mixed mouse primary nerve cell cultures from the cerebral cortex of neonatal mice transgenic for the human PV receptor. These cultures contained all three main cell types of the CNS, i.e. neurons, astrocytes and oligodendrocytes. All three cell types were susceptible to PV infection and virus replication in the cultures led to DNA fragmentation characteristic of apoptosis. PV-induced apoptosis was inhibited by the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD.FMK), indicating that this process involved caspases. Thus, these mixed mouse primary nerve cell cultures are a new <jats:italic>in vitro<\/jats:italic> model for studying the molecular mechanisms of PV-induced apoptosis in nerve cells.<\/jats:p>","DOI":"10.1099\/0022-1317-83-8-1925","type":"journal-article","created":{"date-parts":[[2015,8,26]],"date-time":"2015-08-26T14:46:35Z","timestamp":1440600395000},"page":"1925-1930","source":"Crossref","is-referenced-by-count":26,"title":["An ex vivo murine model to study poliovirus-induced apoptosis in nerve cells"],"prefix":"10.1099","volume":"83","author":[{"given":"Th\u00e9r\u00e8se","family":"Couderc","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Neurovirologie et R\u00e9g\u00e9n\u00e9ration du Syst\u00e8me Nerveux, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, France1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Guivel-Benhassine","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Neurovirologie et 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