{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:56:30Z","timestamp":1791305790621,"version":"4.3.1"},"reference-count":19,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,2,1]]},"abstract":"<jats:p>Thirty-two isolates of <jats:italic>Coxiella burnetii<\/jats:italic> collected from various hosts ranging from arthropods to man were compared by restriction endonuclease (RE) digestion patterns of chromosomal DNA using SDS-PAGE. SDS-PAGE provided better DNA fragment separation than agarose gel electrophoresis and enabled the differentiation of these isolates into six distinct groups on the basis of DNA restriction fingerprints. Two groups of chronic disease isolates could be distinguished, each having unique RE digestion patterns of chromosomal DNA. Three similar but distinct RE digestion patterns were seen among the group of acute disease isolates. Three additional isolates included in this study exhibited a unique RE digestion pattern and also had a unique plasmid type, designated QpDG. DNA-DNA hybridization on selected isolates quantified the relatedness between several groups and supported the classification of these groups as distinct strains.<\/jats:p>","DOI":"10.1099\/00221287-137-2-269","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T04:53:32Z","timestamp":1244177612000},"page":"269-276","source":"Crossref","is-referenced-by-count":103,"title":["Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE"],"prefix":"10.1099","volume":"137","author":[{"given":"Laura R.","family":"Hendrix","sequence":"first","affiliation":[{"name":"Department of Microbiology, Washington State University, Pullman, Washington 99164-4233, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Samuel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis P.","family":"Mallavia","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Washington State University, Pullman, Washington 99164-4233, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/MR.47.2.127-149.1983","article-title":"Q fever and Coxiella burnetii: a model for host-parasite interactions","volume":"47","author":"Baca","year":"1983","journal-title":"Microbiological Reviews"},{"key":"R2","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1128\/JB.115.3.904-911.1973","article-title":"Use of a single-stranded specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes","volume":"115","author":"Crosa","year":"1973","journal-title":"Journal of Bacteriology"},{"key":"R3","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1128\/IAI.52.1.337-340.1986","article-title":"Antigenic variation in the phase I lipopolysac-charide of Coxiella burnetii isolates","volume":"52","author":"Hackstadt","year":"1986","journal-title":"Infection and Immunity"},{"key":"R4","first-page":"2857","article-title":"Active transport of proline by Coxiella burnetii","volume":"130","author":"Hendrix","year":"1984","journal-title":"Journal of General Microbiology"},{"key":"R5","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1128\/JCM.16.3.473-477.1982","article-title":"Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels","volume":"16","author":"Herring","year":"1982","journal-title":"Journal of Clinical Microbiology"},{"key":"R6","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature, London"},{"key":"R7","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1432-1033.1970.tb00830.x","article-title":"The quantitative measurement of DNA hybridization from renaturation rates","volume":"12","author":"deLay","year":"1970","journal-title":"European Journal of Biochemistry"},{"key":"R8","first-page":"67","article-title":"Grouping of Coxiella burnetti isolates by restriction endonuclease analysis","volume":"D79","author":"Mallavia","year":"1985"},{"key":"R9","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Maniatis","year":"1982"},{"key":"R10","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1128\/IAI.45.2.384-389.1984","article-title":"Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis","volume":"45","author":"McClenaghan","year":"1984","journal-title":"Infection and Immunity"},{"key":"R11","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1099\/00207713-30-1-143","article-title":"Genomic relationship among the Typhus group of Rickettsiae","volume":"30","author":"Myers","year":"1980","journal-title":"International Journal of Systematic Bacteriology"},{"key":"R12","doi-asserted-by":"crossref","DOI":"10.7312\/nei-92038","volume-title":"Molecular Evolutionary Genetics","author":"Nei","year":"1987"},{"key":"R13","first-page":"1543","article-title":"Genomic analysis of phase I and II Coxiella burnetii with restriction endonucleases","volume":"131","author":"O\u2019Rourke","year":"1985","journal-title":"Journal of General Microbiology"},{"key":"R14","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1128\/IAI.41.3.1089-1098.1983","article-title":"Serological evaluation of Q fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis","volume":"41","author":"Peacock","year":"1983","journal-title":"Infection and Immunity"},{"key":"R15","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1128\/IAI.41.2.488-493.1983","article-title":"Isolation and characterization of a plasmid from phase I Coxiella burnetii","volume":"41","author":"Samuel","year":"1983","journal-title":"Infection and Immunity"},{"key":"R16","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1128\/IAI.49.3.775-779.1985","article-title":"Correlation of plasmid type and disease caused by Coxiella burnetii","volume":"49","author":"Samuel","year":"1985","journal-title":"Infection and Immunity"},{"key":"R17","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1128\/AEM.51.5.873-884.1986","article-title":"Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics","volume":"51","author":"Selander","year":"1986","journal-title":"Applied and Environmental Microbiology"},{"key":"R18","first-page":"173","article-title":"Q fever. An immunological comparison of strains","volume":"44","author":"Topping","year":"1946","journal-title":"American Journal of Hygiene"},{"key":"R19","first-page":"455","article-title":"Genetic heterogeneity among isolates of Coxiella burnetii","volume":"132","author":"Vodkin","year":"1986","journal-title":"Journal of General Microbiology"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/00221287-137-2-269?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T15:24:07Z","timestamp":1614353047000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/00221287-137-2-269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,2,1]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,2,1]]}},"URL":"https:\/\/doi.org\/10.1099\/00221287-137-2-269","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,2,1]]}}}