{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:49:00Z","timestamp":1762271340605},"reference-count":38,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,2,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Part of a ribosomal ribonucleic acid (rRNA) cistron of <jats:italic>Haemophilus ducreyi<\/jats:italic> was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 <jats:italic>H. ducreyi<\/jats:italic> strains and 13 or 14 non-<jats:italic>H. ducreyi<\/jats:italic> strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for <jats:italic>H. ducreyi<\/jats:italic> strains. Comparisons of 16S rRNA sequences confirm that <jats:italic>H. ducreyi<\/jats:italic> is a member of the <jats:italic>Pasteurellaceae<\/jats:italic> though not closely related to other species in this family.<\/jats:p>","DOI":"10.1099\/00221287-137-2-277","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T04:53:32Z","timestamp":1244177612000},"page":"277-285","source":"Crossref","is-referenced-by-count":23,"title":["The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid"],"prefix":"10.1099","volume":"137","author":[{"given":"Rudi","family":"Rossau","sequence":"first","affiliation":[{"name":"Innogenetics NV, Industriepark 7, Box 4, B-9052 Zwijnaarde, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maddy","family":"Duhamel","sequence":"additional","affiliation":[{"name":"Innogenetics NV, Industriepark 7, Box 4, B-9052 Zwijnaarde, 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