{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T20:03:10Z","timestamp":1782590590120,"version":"3.54.5"},"reference-count":34,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,1,1]]},"abstract":"<jats:p>The upper pathway operon of the toluene catabolic pathway of TOL plasmid pWW0 was shown to carry two open reading frames between the start of transcription and <jats:italic>xylC<\/jats:italic> (encoding benzaldehyde dehydrogenase), the first previously reported gene of the operon. These were designated <jats:italic>xyIUW: xyIU<\/jats:italic> encoded a protein of 131 amino acid residues (<jats:italic>M<jats:sub>r<\/jats:sub>\n                  <\/jats:italic> 14244) which bore no relationship with any protein in the databases, and <jats:italic>xyIW<\/jats:italic> encoded a protein of 348 residues (<jats:italic>M<jats:sub>r<\/jats:sub>\n                  <\/jats:italic> 36992) which was strongly homologous to other long-chain Zn-containing alcohol dehydrogenases. Extracts of <jats:italic>Escherichia coli<\/jats:italic> carrying <jats:italic>xyIUW<\/jats:italic> in expression vector pTrc99A contained a novel protein corresponding to XyIW, but no NAD<jats:sup>+<\/jats:sup> -dependent dehydrogenase activity against benzyl alcohol, mandelate or benzylamine. A mini-Tn5 transposon carrying the meta pathway operon was constructed and from it two strains of <jats:italic>Pseudomonas putida<\/jats:italic> were constructed with the normally plasmid-encoded catabolic operons integrated into the chromosome. Three derivatives of plasmid pKNG101 containing modified <jats:italic>xyIUW<\/jats:italic> genes were constructed, two of which had frameshifts in <jats:italic>xyIU<\/jats:italic> and <jats:italic>xyIW<\/jats:italic>, respectively, and a third with a deletion from the 3\u2032 end of <jats:italic>xyIU<\/jats:italic> into the 5\u2032 end of <jats:italic>xyIW.<\/jats:italic> The wild-type genes of the two <jats:italic>Pseudomonas<\/jats:italic> strains were substituted by the mutant alleles by reverse genetics. The ability of the constructed mutant strains to utilize the aromatic substrates of the TOL pathway was not significantly affected.<\/jats:p>","DOI":"10.1099\/00221287-143-1-101","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T04:53:32Z","timestamp":1244177612000},"page":"101-107","source":"Crossref","is-referenced-by-count":28,"title":["xyIUW, Two Genes at the Start of the upper Pathway Operon of TOL Plasmid Pwwo, Appear to Play no Essential Part in Determining its Catabolic Phenotype"],"prefix":"10.1099","volume":"143","author":[{"given":"Peter A.","family":"Williams","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Wales, Bangor,Gwynedd LL57 2UW,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M.","family":"Shaw","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Wales, Bangor,Gwynedd LL57 2UW,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher W.","family":"Pitt","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Wales, Bangor,Gwynedd LL57 2UW,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milka","family":"Vrecl","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Wales, Bangor,Gwynedd LL57 2UW,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2911(08)60119-8","article-title":"The TOL plasmids-determinants of the catabolism of toluene and the xylenes.","volume":"31","author":"Assinder","year":"1990","journal-title":"Adv Microb Physiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0378-1119(94)00844-I","article-title":"Sequence analysis of the gene cluster encoding toluene-3-monooxygenase from 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