{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:42:17Z","timestamp":1791848537144,"version":"4.3.4"},"reference-count":31,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,9,1]]},"abstract":"<jats:p>Summary: The <jats:italic>omp-31<\/jats:italic> gene, encoding a major outer-membrane protein in <jats:italic>Brucella melitensis<\/jats:italic>, was PCR-amplified from <jats:italic>Brucella<\/jats:italic> strains representing all species and known biovars by using primers selected according to the <jats:italic>B. melitensis<\/jats:italic> 16M <jats:italic>omp-31<\/jats:italic> published sequence. Amplification of <jats:italic>omp-31<\/jats:italic> was achieved from DNA of all <jats:italic>Brucella<\/jats:italic> species with the exception of <jats:italic>Brucella abortus<\/jats:italic>, the only <jats:italic>Brucella<\/jats:italic> species where expression of <jats:italic>omp-31<\/jats:italic> was not detected by reactivity with an mAb specific for an epitope located in Omp-31. Southern blot hybridization of plasmid probes, bearing inserts (4.4-17 kb) containing <jats:italic>B. melitensis<\/jats:italic> 16M <jats:italic>omp-31<\/jats:italic> and adjacent DNA of different sizes, with <jats:italic>Hind<\/jats:italic>III-digested total DNA showed that a large fragment, comprising the entire <jats:italic>omp-31<\/jats:italic> gene and flanking DNA, was actually absent in <jats:italic>B. abortus<\/jats:italic> strains. The size of this DNA fragment has been determined to be about 10 kb. Southern blot hybridization with the different plasmid probes identified species-specific markers for <jats:italic>B. abortus<\/jats:italic> and <jats:italic>B. melitensis<\/jats:italic>. At the biovar level, a specific marker for <jats:italic>B. melitensis<\/jats:italic> bv. 1 was also identified. Additionally, PCR-RFLP studies of <jats:italic>omp-31<\/jats:italic> revealed specific markers for <jats:italic>Brucella ovis, Brucella canis<\/jats:italic> and <jats:italic>Brucella suis<\/jats:italic> bv. 2. Using a combination of <jats:italic>omp-31<\/jats:italic> PCR-RFLP patterns and Southern blot hybridization profiles <jats:italic>Brucella<\/jats:italic> species were differentiated with the sole exception of <jats:italic>Brucella neotomae<\/jats:italic> which was not differentiated from <jats:italic>B. suis<\/jats:italic> bv. 1, 3, 4 and 5. Results presented in this paper demonstrate the potential of <jats:italic>omp-31<\/jats:italic> for differentiating the brucellae and show that <jats:italic>B. abortus<\/jats:italic> lacks a large DNA fragment of about 10 kb containing <jats:italic>omp-31<\/jats:italic> and flanking DNA. In such a large deletion, other genes in addition to <jats:italic>omp-31<\/jats:italic> are probably involved. Sequencing of this DNA fragment will help to identify the missing genes in <jats:italic>B. abortus<\/jats:italic> which could possibly be involved in the differences of pathogenicity and host preference seen in <jats:italic>Brucella<\/jats:italic> species.<\/jats:p>","DOI":"10.1099\/00221287-143-9-2913","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T04:53:32Z","timestamp":1244177612000},"page":"2913-2921","source":"Crossref","is-referenced-by-count":88,"title":["DNA polymorphism at the omp-31 locus of Brucella spp.: evidence for a large deletion in Brucella abortus, and other species-specific markers"],"prefix":"10.1099","volume":"143","author":[{"given":"Nieves","family":"Vizca\u00edno","sequence":"first","affiliation":[{"name":"Laboratoire de Pathologie Infectieuse et Immunologie, Centre de Recherches de Tours, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Verger","sequence":"additional","affiliation":[{"name":"Laboratoire de Pathologie Infectieuse et Immunologie, Centre de Recherches de Tours, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maggy","family":"Grayon","sequence":"additional","affiliation":[{"name":"Laboratoire de Pathologie Infectieuse et Immunologie, Centre de Recherches de Tours, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel S.","family":"Zygmunt","sequence":"additional","affiliation":[{"name":"Laboratoire de Pathologie Infectieuse et Immunologie, Centre de Recherches de Tours, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[{"name":"Laboratoire de Pathologie Infectieuse et Immunologie, Centre de Recherches de Tours, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"4603","DOI":"10.1128\/JB.170.10.4603-4607.1988","article-title":"DNA polymorphism in strains of the genus Brucella","volume":"170","author":"Allardet-Servent","year":"1988","journal-title":"J Bacteriol"},{"key":"R2","volume-title":"Techniques for the Brucellosis Laboratory.","author":"Alton","year":"1988"},{"key":"R3","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/JCM.32.11.2660-2666.1994","article-title":"Differentiation of Brucellaabortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucellasuis bv. 1 by 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