{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T15:58:10Z","timestamp":1772812690224,"version":"3.50.1"},"reference-count":33,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,4,1]]},"abstract":"<jats:p>The intergenic spacer region between the <jats:italic>rrs<\/jats:italic> and <jats:italic>rrl<\/jats:italic> ribosomal RNA genes of <jats:italic>Haemophilus ducreyi<\/jats:italic> was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of <jats:italic>H. ducreyi<\/jats:italic> was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. 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