{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:36:04Z","timestamp":1790980564997,"version":"4.1.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p><jats:italic>Brucella abortus<\/jats:italic>is known to produce 2,3-dihydroxybenzoate (2,3-DHBA) and to use this catechol as a siderophore to grow under iron-limited conditions. In this study a mutant (BAM41) is described that is deficient in siderophore production by insertion of Tn<jats:italic>5<\/jats:italic>in the virulent<jats:italic>B. abortus<\/jats:italic>strain 2308. This mutant was unable to grow on iron-deprived medium and its growth could not be restored by addition of 2,3-DHBA. Production of catecholic compounds by both the<jats:italic>Brucella<\/jats:italic>mutant and parental strains under iron-deprivation conditions was assayed by TLC. Two catecholic substances were identified in the supernatant of the parental strain 2308. The faster migrating spot showed the same retention factor (<jats:italic>R<\/jats:italic><jats:sub>f<\/jats:sub>) as that of purified 2,3-DHBA. The mutant BAM41 overproduced 2,3-DHBA, but failed to form the slower migrating catechol. This defect could only be complemented by the addition of the slow-migrating catechol from strain 2308. The genomic region containing Tn<jats:italic>5<\/jats:italic>in BAM41 was cloned and the position of the transposon was determined by nucleotide sequencing. The sequence revealed that the insertion had occurred at a gene with homology to<jats:italic>Escherichia coli entF<\/jats:italic>, a locus involved in the late steps of the biosynthesis of the complex catecholic siderophore enterobactin. Intracellular survival and growth rates of the<jats:italic>B. abortus<\/jats:italic>wild-type and<jats:italic>entF<\/jats:italic>mutant strains in mouse-derived J774 macrophages were similar, indicating that production of this siderophore was not essential in this model of infection. It is concluded that<jats:italic>B. abortus<\/jats:italic>synthesizes a previously unknown and highly efficient catecholic siderophore, different from 2,3-DHBA, for which the name brucebactin is proposed.<\/jats:p>","DOI":"10.1099\/00221287-148-2-353","type":"journal-article","created":{"date-parts":[[2015,8,26]],"date-time":"2015-08-26T15:40:10Z","timestamp":1440603610000},"page":"353-360","source":"Crossref","is-referenced-by-count":52,"title":["Brucella abortus strain 2308 produces brucebactin, a highly efficient catecholic siderophore The GenBank accession number for the sequence reported in this paper is AF361942."],"prefix":"10.1099","volume":"148","author":[{"given":"Manuel I","family":"Gonz\u00e1lez Carrer\u00f3","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u0131\u0301a Molecular (Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas, CSIC), Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s\/n, 39011-Santander, Spain1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9lix J","family":"Sangari","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u0131\u0301a Molecular (Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas, CSIC), Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s\/n, 39011-Santander, Spain1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Ag\u00fcero","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u0131\u0301a Molecular (Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas, CSIC), Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s\/n, 39011-Santander, Spain1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M","family":"Garc\u0131\u0301a Lobo","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u0131\u0301a Molecular (Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas, 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