{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,14]],"date-time":"2026-11-14T08:37:25Z","timestamp":1794645445692,"version":"4.9.1"},"reference-count":44,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p>Several independent gene clusters containing varying lengths of type I polyketide synthase genes were isolated from <jats:italic>\u2018Streptomyces nanchangensis\u2019<\/jats:italic> NS3226, a producer of nanchangmycin and meilingmycin. The former is a polyether compound similar to dianemycin and the latter is a macrolide compound similar to milbemycin, which shares the same macrolide ring as avermectin but has different side groups. Clusters A\u2013H spanned about 133, 132, 104, 174, 122, 54, 37 and 59\u00a0kb, respectively. Two systems were developed for functional analysis of the gene clusters by gene disruption or replacement. (1) <jats:italic>Streptomyces<\/jats:italic> phage \u03d5C31 and its derived vectors can infect and lysogenize this strain. (2) pSET152, an <jats:italic>Escherichia coli<\/jats:italic> plasmid with \u03d5C31 <jats:italic>attP<\/jats:italic> site, and pHZ1358, a <jats:italic>Streptomyces\u2013Escherichia coli<\/jats:italic> shuttle cosmid vector, both carrying <jats:italic>oriT<\/jats:italic> from RP4, can be mobilized from <jats:italic>E. coli<\/jats:italic> into NS3226 by conjugation. pHZ1358 was shown to be generally useful for generating mutant strains by gene disruption and replacement in NS3226 as well as in several other <jats:italic>Streptomyces<\/jats:italic> strains. A region in cluster A (\u223c133\u00a0kb) seemed to be involved in nanchangmycin production because replacement of several DNA fragments in this region by an apramycin resistance gene [<jats:italic>aac3(IV)<\/jats:italic>] gave rise to nanchangmycin non-producing mutants.<\/jats:p>","DOI":"10.1099\/00221287-148-2-361","type":"journal-article","created":{"date-parts":[[2015,8,26]],"date-time":"2015-08-26T15:39:27Z","timestamp":1440603567000},"page":"361-371","source":"Crossref","is-referenced-by-count":126,"title":["\u2018Streptomyces nanchangensis\u2019, a producer of the insecticidal polyether antibiotic nanchangmycin and the antiparasitic macrolide meilingmycin, contains multiple polyketide gene clusters"],"prefix":"10.1099","volume":"148","author":[{"given":"Yuhui","family":"Sun","sequence":"first","affiliation":[{"name":"Jiangxi Agricultural University, Nanchang 330045, China3"},{"name":"Huazhong Agricultural University, Wuhan 430070, China2"},{"name":"Bio-X Life Science Research 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