{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:07:47Z","timestamp":1747181267970,"version":"3.40.5"},"reference-count":23,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,1]]},"abstract":"\n Background.<\/jats:bold>\n \n \n Staphylococcus aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is a common cause of nosocomial infections. Epidemiological typing of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> enables control of its spread. The objective of this study was to investigate coagulase gene polymorphisms of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolated from patients at Kosti Hospital in Sudan.<\/jats:p>\n \n Methods.<\/jats:bold> In total, 110 \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates were recovered from 110 patients who were enrolled in the study. \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains were isolated on blood agar and MacConkey agar and then identified by conventional tests. Resistance to methicillin was determined by detection of the mecA<\/jats:italic> gene. Polymorphism in the coagulase gene (coa<\/jats:italic>) was investigated using PCR followed by Alu<\/jats:italic>I RFLP analysis.<\/jats:p>\n \n Results.<\/jats:bold> Methicillin-resistant \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> accounted for 62\/110 (56 %) of the isolates. PCR of the coa<\/jats:italic> gene showed two different amplicons, one of 680 bp detected in 83\/110 (75.5 %) of the isolates and one of 790 bp detected in 27\/110 (24.5 %). When digested with the Alu<\/jats:italic>I enzyme, the 790 bp amplicon resulted in three restriction fragments of 500, 210 and 80 bp (coa<\/jats:italic>1). Restriction of the 680 bp amplicon gave two patterns; the first (coa<\/jats:italic>2) was found in 22\/110 (20 %) of the isolates and showed four fragments of 210, 210, 180 and 80 bp, and the second (coa<\/jats:italic>3) was found in 61\/110 (55.5 %) and revealed three fragments of 390, 210 and 80 bp. Most of the coa<\/jats:italic>3 isolates (75.4%) were methicillin-resistant.<\/jats:p>\n \n Conclusion.<\/jats:bold> Three polymorphic genotypes of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were identified in patients at Kosti Hospital. The coa<\/jats:italic>3 genotype was the predominant one and was mostly detected in methicillin-resistant isolates.<\/jats:p>","DOI":"10.1099\/acmi.0.000026","type":"journal-article","created":{"date-parts":[[2019,5,15]],"date-time":"2019-05-15T16:13:31Z","timestamp":1557936811000},"source":"Crossref","is-referenced-by-count":4,"title":["Coagulase gene polymorphisms of Staphylococcus aureus isolates from patients at Kosti Teaching Hospital, Sudan"],"prefix":"10.1099","volume":"1","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8112-3461","authenticated-orcid":false,"given":"Omer Mohammed Ali","family":"Ibrahim","sequence":"first","affiliation":[{"name":"1 Department of Microbiology, Faculty of Medical Laboratory Sciences, University of El-Imam El-Mahdi, Kosti, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naser Eldin","family":"Bilal","sequence":"additional","affiliation":[{"name":"2 Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed E. 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