{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:26:59Z","timestamp":1781072819861,"version":"3.54.1"},"reference-count":39,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:p>The mouse (<jats:italic>Mus musculus<\/jats:italic>) has been extensively used for studying brucellosis, regarding pathogenesis, immunity and the evaluation of vaccines and therapeutics. In this work, RNA-seq was applied to explore the immunological potential of a live <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1382\" xlink:type=\"simple\">Brucella abortus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> S19\u2206<jats:italic>per<\/jats:italic>, a perosamine synthetase gene mutant of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1382\" xlink:type=\"simple\">B. abortus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> S19. Comparison of transcriptome data was carried out for identifying differentially expressed genes among PBS (control) and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1382\" xlink:type=\"simple\">B. abortus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> S19\u2206<jats:italic>per<\/jats:italic> immunized mice at 15 days post-immunization. Functional analysis revealed 545 significant differentially expressed genes related to mouse immunity. Specific activation of MHC-I and MHC-II antigen-processing pathways were identified as the highly enriched pathways based on Kyoto Encyclopedia of Genes and Genomes annotation. Other major immune response pathways regulated within the host were NF-kappa B signalling, chemokine signalling, T-cell receptor pathway, apoptosis, TNF signalling and nucleotide-binding oligomerization domain-like receptor signalling. These data provided new insights into the molecular mechanisms of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1382\" xlink:type=\"simple\">B. abortus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> S19\u2206<jats:italic>per<\/jats:italic>-induced immune response in mice spleen that might facilitate the development of a highly immunogenic vaccine against brucellosis.<\/jats:p>","DOI":"10.1099\/acmi.0.000082","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T17:50:42Z","timestamp":1575309042000},"source":"Crossref","is-referenced-by-count":4,"title":["Transcriptome analysis of Brucella abortus S19\u2206per immunized mouse spleen revealed activation of MHC-I and MHC-II pathways"],"prefix":"10.1099","volume":"2","author":[{"given":"Khushal Singh","family":"Solanki","sequence":"first","affiliation":[{"name":"Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9424-1902","authenticated-orcid":false,"given":"Ravi Kumar","family":"Gandham","sequence":"additional","affiliation":[{"name":"National Institute of Animal Biotechnology, Opp. Journalist Colony, Near Gowlidoddy Extended, Q City Road, Gachibowli, Hyderabad, Telangana 500032, India"},{"name":"Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasad","family":"Thomas","sequence":"additional","affiliation":[{"name":"Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallab","family":"Chaudhuri","sequence":"additional","affiliation":[{"name":"Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.03.013"},{"key":"R2","first-page":"1","article-title":"Basic Animal Husbandry Statistics. 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