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To further investigate the role of various blood components in prion disease transmission, we intracranially inoculated genetically susceptible VRQ\/ARQ and ARQ\/ARQ sheep with inocula composed of CD11c<jats:sup>+<\/jats:sup>B1 lymphocytes, CD68 +macrophages, or platelet-rich plasma derived from clinically ill sheep infected with the US no. 13\u20137 scrapie agent. At the completion of the study, we found that VRQ\/ARQ and ARQ\/ARQ sheep inoculated with CD11c<jats:sup>+<\/jats:sup>B1 lymphocytes and CD68<jats:sup>+<\/jats:sup>macrophages developed scrapie with detectable levels of PrP<jats:sup>Sc<\/jats:sup>in the central nervous system and lymphoreticular system, while those inoculated with platelet-rich plasma did not develop disease and did not have detectable PrP<jats:sup>Sc<\/jats:sup>by immunohistochemistry or enzyme immunoassay. This study complements and expands on earlier findings that white blood cells harbour prion infectivity, and reports CD11c<jats:sup>+<\/jats:sup>B1 lymphocytes and CD68<jats:sup>+<\/jats:sup>macrophages as additional targets for possible preclinical detection of prion infection in blood.<\/jats:p>","DOI":"10.1099\/acmi.0.000155","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T17:19:07Z","timestamp":1595956747000},"source":"Crossref","is-referenced-by-count":0,"title":["Experimental inoculation of CD11c+ B1 lymphocytes, CD68+ macrophages, or platelet-rich plasma from scrapie-infected sheep into susceptible sheep results in variable infectivity"],"prefix":"10.1099","volume":"2","author":[{"given":"Najiba","family":"Mammadova","sequence":"first","affiliation":[{"name":"Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). 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