{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T03:57:26Z","timestamp":1771991846964,"version":"3.50.1"},"reference-count":59,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Health and Medical Research Centre","award":["APP1023239"],"award-info":[{"award-number":["APP1023239"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,1]]},"abstract":"<jats:p>Urogenital <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7767\" xlink:type=\"simple\">Chlamydia trachomatis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> infection is the most common sexually transmitted bacterial infection throughout the world. While progress has been made to better understand how type strains develop and respond to environmental stress <jats:italic>in vitro<\/jats:italic>, very few studies have examined how clinical isolates behave under similar conditions. Here, we examined the development and persistence phenotypes of several clinical isolates, to determine how similar they are to each other, and the type strain <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7767\" xlink:type=\"simple\">C. trachomatis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> D\/UW-3\/Cx. The type strain was shown to produce infectious progeny at a higher magnitude than each of the clinical isolates, in each of the six tested cell lines. All chlamydial strains produced the highest number of infectious progeny at 44\u2009h post-infection in the McCoy B murine fibroblast cell line, yet showed higher levels of infectivity in the MCF-7 human epithelial cell line. The clinical isolates were shown to be more susceptible than the type strain to the effects of penicillin and iron deprivation persistence models in the MCF-7 cell line. While subtle differences between clinical isolates were observed throughout the experiments conducted, no significant differences were identified. This study reinforces the importance of examining clinical isolates when trying to relate <jats:italic>in vitro<\/jats:italic> data to clinical outcomes, as well as the importance of considering the adaptations many type strains have to being cultured <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/acmi.0.000204","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T15:18:42Z","timestamp":1613747922000},"source":"Crossref","is-referenced-by-count":4,"title":["Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro"],"prefix":"10.1099","volume":"3","author":[{"given":"Mark","family":"Thomas","sequence":"first","affiliation":[{"name":"Institute of Health and Biomedical Innovation, Faculty of Health, Queensland University of Technology, Kelvin Grove, Queensland, Australia"},{"name":"School of Life Sciences, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2948-1648","authenticated-orcid":false,"given":"Amba","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, Faculty of Health, Queensland University of Technology, Kelvin Grove, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6644-3846","authenticated-orcid":false,"given":"Samuel","family":"Kroon","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenka A.","family":"Vodstrcil","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Biostatistics, Melbourne School of Population and Global Health, University of Melbourne, Parkville, Victoria, Australia"},{"name":"Melbourne Sexual Health Centre, Alfred Hospital, Carlton, Victoria, Australia"},{"name":"Central Clinical School, Monash University, Melbourne, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2549-8111","authenticated-orcid":false,"given":"Samuel","family":"Phillips","sequence":"additional","affiliation":[{"name":"Murdoch Childrens Research Institute, Parkville 3052, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane S.","family":"Hocking","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Biostatistics, Melbourne School of Population and Global Health, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1682-0025","authenticated-orcid":false,"given":"Peter","family":"Timms","sequence":"additional","affiliation":[{"name":"Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0879-1287","authenticated-orcid":false,"given":"Wilhelmina M.","family":"Huston","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, Faculty of Health, Queensland University of Technology, Kelvin Grove, Queensland, Australia"},{"name":"School of Life Sciences, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","volume-title":"Biomathematical Modeling of Chlamydia Infection and Disease. 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