{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T03:50:50Z","timestamp":1752983450782,"version":"3.40.5"},"reference-count":60,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,22]]},"abstract":"<jats:p>Methane emissions from enteric fermentation in the ruminant digestive system generated by methanogenic archaea are a significant contributor to anthropogenic greenhouse gas emissions. Additionally, methane produced as an end-product of enteric fermentation is an energy loss from digested feed. To control the methane emissions from ruminants, extensive research in the last decades has been focused on developing viable enteric methane mitigation practices, particularly, using methanogen-specific inhibitors. We report here the utilization of two known inhibitors of methanogenic archaea, neomycin and chloroform, together with a recently identified inhibitor, echinomycin, to produce resistant mutants of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.155\" xlink:type=\"simple\">Methanococcus maripaludis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> S2 and S0001. Whole-genome sequencing at high coverage (&gt; 100-fold) was performed subsequently to investigate the potential targets of these inhibitors at the genomic level. Upon analysis of the whole-genome sequencing data, we identified mutations in a number of genetic loci pointing to potential mechanisms of inhibitor action and their underlying mechanisms of resistance.<\/jats:p>","DOI":"10.1099\/acmi.0.000244","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T15:15:43Z","timestamp":1626880543000},"source":"Crossref","is-referenced-by-count":4,"title":["Using genome comparisons of wild-type and resistant mutants of Methanococcus maripaludis to help understand mechanisms of resistance to methane inhibitors"],"prefix":"10.1099","volume":"3","author":[{"given":"Feng","family":"Long","sequence":"first","affiliation":[{"name":"Present address: Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH, USA"},{"name":"Department of Microbiology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5850-0919","authenticated-orcid":false,"given":"Chen-Yi","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1229-0423","authenticated-orcid":false,"given":"William B.","family":"Whitman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory M.","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron S.","family":"Ronimus","sequence":"additional","affiliation":[{"name":"Rumen Microbiology, AgResearch Ltd., Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1600298113","article-title":"Mode of action uncovered for the specific reduction of methane emissions from ruminants by the small molecule 3-nitrooxypropanol","volume":"113","author":"Duin","year":"2016","journal-title":"P Natl Acad Sci USA"},{"key":"R2","first-page":"659","volume-title":"Climate Change 2013: The Physical Science Basis. 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