{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:48:46Z","timestamp":1758124126994,"version":"3.40.5"},"reference-count":13,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,18]]},"abstract":"<jats:p>The QuickGene-810 Nucleic Acid Isolation System is a semi-automated extraction platform which may be used for RNA extraction. New methods were required to support the rapid increase in respiratory virus testing during the SARS-CoV-2 pandemic. The aim of this study was to assess SARS-CoV-2 RNA extraction using the QuickGene-810 kit compared to the EZ1 Advanced Extraction Platform for use on the AusDiagnostics SARS-CoV-2, Influenza and RSV 8-well RT-PCR assay. Qualitative results from all clinical samples were concordant between the QuickGene-810 and the EZ1 extraction methods, demonstrating that the QuickGene-810 kit is suitable for use in pathogen diagnostics. However, there was an average difference of approximately two cycles between the cycle threshold (Ct) values for both SARS-CoV-2 targets, suggesting that the EZ1 kit yields a higher concentration of nucleic acid extract, possibly related to its use of carrier RNA and\/or smaller elution volume, which infers the possibility of false negative results for samples with very low viral loads.<\/jats:p>","DOI":"10.1099\/acmi.0.000353","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T15:16:29Z","timestamp":1653664589000},"source":"Crossref","is-referenced-by-count":2,"title":["A comparison of SARS-CoV-2 RNA extraction with the QuickGene-810 Nucleic Acid Isolation System compared to the EZ1 Advanced DSP Virus Kit"],"prefix":"10.1099","volume":"4","author":[{"given":"Thuy","family":"Phan","sequence":"first","affiliation":[{"name":"NSW Health Pathology, Department of Microbiology and Infectious Diseases, Concord Repatriation and General Hospital, Hospital Rd, Concord, NSW 2139, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Stephenson","sequence":"additional","affiliation":[{"name":"NSW Health Pathology, Department of Microbiology and Infectious Diseases, Concord Repatriation and General Hospital, Hospital Rd, Concord, NSW 2139, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina","family":"Cai","sequence":"additional","affiliation":[{"name":"NSW Health Pathology, Department of Microbiology and Infectious Diseases, Concord Repatriation and General Hospital, Hospital Rd, Concord, NSW 2139, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikol","family":"Andacic","sequence":"additional","affiliation":[{"name":"NSW Health Pathology, Department of Microbiology and Infectious Diseases, Concord Repatriation and General Hospital, Hospital Rd, Concord, NSW 2139, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5238-9260","authenticated-orcid":false,"given":"Genevieve","family":"McKew","sequence":"additional","affiliation":[{"name":"Concord Clinical School, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia"},{"name":"NSW Health Pathology, Department of Microbiology and Infectious Diseases, Concord Repatriation and General Hospital, Hospital Rd, Concord, NSW 2139, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"journal-title":"Figshare","year":"2020","author":"Phan","key":"R1"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-021-00360-w"},{"year":"2021","key":"R3","article-title":"Supply Shortages Impacting COVID-19 and Non-COVID tTesting"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/srep45199"},{"volume-title":"Concord Hospital Microbiology","year":"2020","author":"Phan","key":"R5"},{"first-page":"1","volume-title":"AusDiagnostics High-Plex 24 System REF 91501. 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