{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:11:22Z","timestamp":1788909082036,"version":"build-2803163510"},"reference-count":28,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,18]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1678\" xlink:type=\"simple\">Ralstonia mannitolilytica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> is a rare opportunistic pathogen capable of causing a serious infection in immunocompromised patients. Our objective was to describe all cases of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1678\" xlink:type=\"simple\">R. mannitolilytica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> bloodstream infection identified within 2\u2009years at our tertiary care centre, focusing on clinical characteristics, risk factors, antibiotic sensitivity patterns, management and outcomes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Case Series.<\/jats:bold> We compiled a descriptive case series including 14 non-duplicate <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1678\" xlink:type=\"simple\">R. mannitolilytica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates obtained from bloodstream infection samples from the microbiology laboratory of a tertiary care centre from June 2019 to June 2021. All isolates were initially identified based on their morphological properties and biochemical reactions, and then underwent matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) examination for confirmation of identity. Antibiotic susceptibility testing was performed using the Kirby\u2013Bauer disc diffusion method and Vitek 2. All 14\u2009patients presented with symptoms of fever and\/or chills, and a positive blood culture for <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1678\" xlink:type=\"simple\">R. mannitolilytica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. After 48\u2009h of incubation, no <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1671\" xlink:type=\"simple\">Ralstonia<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> growth was reported from any of the current environmental or pharmaceutical water samples. Chemotherapy (9\/14), mechanical ventilation (4\/14), steroid use (2\/14) and diabetes mellitus (1\/14) were associated risk factors in our patients. The antibiotic sensitivity panel showed maximum resistance to aminoglycosides (64.3%) and no resistance to cefoperazone\/sulbactum. Patients received treatment with cefoperazone\/sulbactum and meropenem or ceftazidime. Thirteen patients recovered with antibiotic therapy and one patient succumbed to his illness.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1678\" xlink:type=\"simple\">R. mannitolilytica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> can cause bloodstream infections in immunocompromised patients. It is likely to be missed or underreported due to lack of clinical awareness. MALDI-TOF MS is helpful in rapid identification. <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1678\" xlink:type=\"simple\">R. mannitolilytica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> is resistant to many routinely used antibiotics, including carbapenems.<\/jats:p>","DOI":"10.1099\/acmi.0.000367","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T17:16:16Z","timestamp":1654017376000},"source":"Crossref","is-referenced-by-count":15,"title":["Ralstonia mannitolilytica: an emerging multidrug-resistant opportunistic pathogen in a tertiary care hospital setting"],"prefix":"10.1099","volume":"4","author":[{"given":"Tasneem","family":"Siddiqui","sequence":"first","affiliation":[{"name":"Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangram Singh","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6380-6488","authenticated-orcid":false,"given":"Richa","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ujjala","family":"Ghoshal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5440-7717","authenticated-orcid":false,"given":"Chinmoy","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-1975-9"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/S0255-0857(21)03017-6"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-017-0178-z"},{"key":"R4","first-page":"374","article-title":"Ralstonia mannitolilytica bacteraemia: a case report and literature review","volume":"26","author":"Boattini","year":"2018","journal-title":"Infez Med"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.5867\/medwave.2021.04.8200"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keaa830"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1186\/s13256-019-2235-0"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-021-04214-5"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.2174\/1871526520666200330163504"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1177\/00494755211036557"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.4103\/1319-2442.261367"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.idcr.2020.e01003"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00013-14"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S206492"},{"key":"R15","volume-title":"Mackie & McCartney Practical Medical Microbiology","author":"Collee","year":"1996"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-2-547"},{"key":"R17","article-title":"VITEK EXPANDED DATABASE MS Now with Mycobacteria, Nocardia and Moulds","year":"2016"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.8.2964-2966.2001"},{"key":"R19","volume-title":"Performance standards for antimicrobial susceptibility testing. 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