{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T07:02:22Z","timestamp":1771657342128,"version":"3.50.1"},"reference-count":46,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Group B <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5605\" xlink:type=\"simple\">Streptococcus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (GBS) is a human commensal bacterium that is also associated with infection in pregnant and non-pregnant adults, neonates and elderly people.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap Statement.<\/jats:bold> The authors hypothesize that knowledge of regional GBS genetic patterns may allow the use of prevention and treatment measures to reduce the burden of streptococcal disease.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> The aim was to report the genotypic diversity and antimicrobial sensitivity profiles of invasive, noninvasive urinary and colonizing GBS strains, and evaluate the relationships between these findings.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> The study included consecutive and non-duplicated GBS isolates recovered in southern Brazil from 2015 to 2017. We performed multiple-locus variable-number tandem repeat analysis (MLVA) and PCR analyses to determine capsular serotypes and identify the presence of the resistance genes <jats:italic>mef<\/jats:italic>A\/E, <jats:italic>erm<\/jats:italic>B and <jats:italic>erm<\/jats:italic>A\/TR, and also antibiotic susceptibility testing.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The sample consisted of 348 GBS strains, 42 MLVA types were identified, and 4 of them represented 64\u200a% of isolates. Serotype Ia was the most prevalent (42.2\u200a%) and was found in a higher percentage associated with colonization, followed by serotypes V (24.4\u200a%), II (17.8\u200a%) and III (7.8\u200a%). Serotype V was associated with invasive isolates and serotypes II and III with noninvasive isolates, without significant differences. All isolates were susceptible to penicillin. GBS 2018\/ <jats:italic>hvg<\/jats:italic>A was observed in 17 isolates, with 11 belonging to serogroup III. The Hunter\u2013Gaston diversity index was calculated as 0.879. The genes <jats:italic>mef<\/jats:italic>A\/E, <jats:italic>erm<\/jats:italic>\/B and <jats:italic>erm<\/jats:italic>\/A\/TR were found in 45, 19 and 46 isolates.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> This report suggests that the circulating GBS belong to a limited number of genetic lineages. The most common genotypes were Ia\/MT12 and V\/MT18, which are associated with high resistance to macrolides and the presence of the genes <jats:italic>mef<\/jats:italic>A\/E and <jats:italic>erm<\/jats:italic>A\/TR. Penicillin remains the antibiotic of choice. Implementation of continuous surveillance of GBS infections will be essential to assess GBS epidemiology and develop accurate GBS prevention, especially strategies associated with vaccination.<\/jats:p>","DOI":"10.1099\/acmi.0.000370","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T14:18:49Z","timestamp":1654093129000},"source":"Crossref","is-referenced-by-count":5,"title":["Genetic diversity and antimicrobial resistance of invasive, noninvasive and colonizing group B Streptococcus isolates in southern Brazil"],"prefix":"10.1099","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7561-4790","authenticated-orcid":false,"given":"Otto Henrique May","family":"Feuerschuette","sequence":"first","affiliation":[{"name":"Universidade do Sul de Santa Catarina \u2013 UNISUL, Tubar\u00e3o, Brazil"},{"name":"Hospital Universit\u00e1rio Professor Polydoro Ernani de S\u00e3o Thiago, HU\/UFSC, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Ven\u00e2ncio","family":"Alves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Microbiologia e Sorologias, HU\/UFSC, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2735-5197","authenticated-orcid":false,"given":"Mara Cristina","family":"Scheffer","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Microbiologia e Sorologias, HU\/UFSC, Florian\u00f3polis, Brazil"},{"name":"Hospital Universit\u00e1rio Professor Polydoro Ernani de S\u00e3o Thiago, HU\/UFSC, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9030-0688","authenticated-orcid":false,"given":"Ana Paula Pessoa","family":"Vilela","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Microbiologia e Sorologias, HU\/UFSC, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Hartmann","family":"Barazzetti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Microbiologia e Sorologias, HU\/UFSC, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique Miranda","family":"Feuerschuette","sequence":"additional","affiliation":[{"name":"Universidade do Sul de Santa Catarina \u2013 UNISUL, Tubar\u00e3o, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina Lobor","family":"Cancelier","sequence":"additional","affiliation":[{"name":"Universidade do Sul de Santa Catarina \u2013 UNISUL, Tubar\u00e3o, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1292-0974","authenticated-orcid":false,"given":"Maria Luiza","family":"Bazzo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Microbiologia e Sorologias, HU\/UFSC, Florian\u00f3polis, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.61.3.335"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5544"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.01.009"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix664"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2018.7269"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007421"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0007-2018"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02417-12"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2014.11.001"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-171"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.2807\/ese.18.04.20380-en"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00728-19"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00234-10"},{"key":"R14","first-page":"1","article-title":"Division of bacterial diseases, national center for immunization and respiratory diseases, centers for disease control and prevention (CDC). prevention of perinatal group B streptococcal disease-revised guidelines from CDC, 2010","volume":"59","author":"Verani","year":"2010","journal-title":"MMWR Recomm Rep"},{"key":"R15","volume-title":"Performance standards for antimicrobial susceptibility testing. 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