{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T23:41:58Z","timestamp":1740181318611,"version":"3.37.3"},"reference-count":21,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T00:00:00Z","timestamp":1666915200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["213979\/Z\/18\/Z"],"award-info":[{"award-number":["213979\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010330","name":"Anglia Ruskin University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010330","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010330","name":"Anglia Ruskin University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010330","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,28]]},"abstract":"<jats:p>The OXA \u03b2-lactamases are responsible for hydrolysing \u03b2-lactam antibiotics and contribute to the multidrug-resistant phenotype of several major human pathogens. The OXA<jats:italic>Ab<\/jats:italic> enzymes are intrinsic to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2767\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and can confer resistance to carbapenem antibiotics. Here we determined the structure of the most prevalent OXA<jats:italic>Ab<\/jats:italic> enzyme, OXA-66. The structure of OXA-66 was solved at a resolution of 2.1\u2009\u00c5 and found to be very similar to the structure of OXA-51, the only other OXA<jats:italic>Ab<\/jats:italic> enzyme that has had its structure solved. Our data contained one molecule per asymmetric unit, and analysis of positions responsible for dimer formation in other OXA enzymes suggest that OXA-66 likely exists as a monomer.<\/jats:p>","DOI":"10.1099\/acmi.0.000412","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T17:19:50Z","timestamp":1664817590000},"source":"Crossref","is-referenced-by-count":0,"title":["OXA-66 structure and oligomerisation of OXAAb enzymes"],"prefix":"10.1099","volume":"4","author":[{"given":"Yuiko","family":"Takebayashi","sequence":"first","affiliation":[{"name":"School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK"},{"name":"Department of Biomedical and Forensic Science, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara R.","family":"Henderson","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK"},{"name":"Norwich Medical School, University of East Anglia, Norwich, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitri Y.","family":"Chirgadze","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip J.","family":"Warburton","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health, University of Plymouth, Plymouth, UK"},{"name":"Department of Biomedical and Forensic Science, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6849-9758","authenticated-orcid":false,"given":"Benjamin A.","family":"Evans","sequence":"additional","affiliation":[{"name":"Norwich Medical School, University of East Anglia, Norwich, UK"},{"name":"Department of Biomedical and Forensic Science, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00117-13"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1101\/2020.07.02.183822"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5b00090"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0224303"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607557104"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.04.010"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14643"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-4838(01)00133-9"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.280115"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/10.1.1"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7015-5_6"},{"year":"2010","key":"R12","article-title":"PROTEUM 2 User Manual"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911007281"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01096.x"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3040"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4805"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/79688"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.6b00660"}],"container-title":["Access Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/acmi\/10.1099\/acmi.0.000412?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T18:25:30Z","timestamp":1702578330000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/acmi\/10.1099\/acmi.0.000412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,28]]},"references-count":21,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10,28]]}},"URL":"https:\/\/doi.org\/10.1099\/acmi.0.000412","relation":{},"ISSN":["2516-8290"],"issn-type":[{"type":"electronic","value":"2516-8290"}],"subject":[],"published":{"date-parts":[[2022,10,28]]}}}