{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:56:35Z","timestamp":1790906195294,"version":"4.1.0"},"reference-count":38,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01AI132668"],"award-info":[{"award-number":["R01AI132668"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RGPIN-2022-04026"],"award-info":[{"award-number":["RGPIN-2022-04026"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Vanier Canada Graduate Scholarship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:p>\n                    West Nile virus (WNV) is the most prevalent mosquito-borne virus and the leading cause of viral encephalitis in the continental United States. It belongs to the family\n                    <jats:italic>Flaviviridae<\/jats:italic>\n                    which includes other important human pathogens such as dengue virus (DENV), Japanese encephalitis virus (JEV) and Zika viruses (ZIKV). Despite several decades of research, no specific antiviral drugs are available to treat flavivirus infections. The present study characterizes the interaction between the WNV NS3 and NS5 proteins for the purpose of identifying hotspots in the protein-protein interaction which could be targeted for the development of antiviral therapeutics. We previously developed an interaction model\n                    <jats:italic>in silico<\/jats:italic>\n                    based on data available in the literature. Here, potential interacting residues on NS3 and NS5 were mutated in a WNV replicon, and seven mutations in the NS3 protein were found to drastically reduce viral replication. In addition to being well conserved among mosquito-borne flaviviruses, these residues are located on the protein\u2019s surface in two clusters which might be interesting new targets for future drug development.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.000675.v3","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T06:20:39Z","timestamp":1719382839000},"source":"Crossref","is-referenced-by-count":7,"title":["Deciphering the interaction surface between the West Nile virus NS3 and NS5 proteins"],"prefix":"10.1099","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4048-746X","authenticated-orcid":false,"given":"Carolin","family":"Brand","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biochimie et de G\u00e9nomique Fonctionnelle, Universit\u00e9 de Sherbrooke, Sherbrooke, QC, 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