{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T02:41:21Z","timestamp":1772764881851,"version":"3.50.1"},"reference-count":25,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia Autonomous Region","doi-asserted-by":"crossref","award":["2021ZD16"],"award-info":[{"award-number":["2021ZD16"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Inner Mongolia Medical and Health Science and Technology Plan","award":["202202056"],"award-info":[{"award-number":["202202056"]}]},{"name":"The Inner Mongolia Medical and Health Science and Technology Plan","award":["202202161"],"award-info":[{"award-number":["202202161"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:p>\n                    <jats:bold>Abstract<\/jats:bold>\n                  <\/jats:p>\n                  <jats:p>\n                    The objective is to analyse the physicochemical properties, spatial structure and protein\u2013protein interactions (PPIs) of L7\/L12 protein using bioinformatics methods and predict their B- and T-cell epitopes to lay a theoretical foundation for developing a novel multiepitope vaccine (MEV). The National Center for Biotechnology Information (NCBI) database was searched for the amino acid sequences of L7\/L12 from\n                    <jats:italic>Brucella melitensis<\/jats:italic>\n                    . In addition, the online softwares, ProtParam and ProtScale, were used to predict the physicochemical properties: NetPhos3.1 and CD-search to predict the phosphorylation sites and conserved domains; SOMPA and SWISS-MODEL to predict the secondary and tertiary structures; the STRING database to analyse the PPIs; and the IEDB, ABCpred, SVMTrip and SYFPEITHI databases to predict the B- and T-cell epitopes. L7\/L12 was docked to Toll-like receptor 4 (TLR4), B-cell receptor (BCR), Major histocompatibility complex I-T cell receptor (MHC I-TCR) and MHC II-TCR complexes, respectively, and the binding ability of L7\/L12 to the targeted receptors was tested. L7\/L12, consisting of 124 amino acids, was determined to be a stable, intracellular, hydrophilic protein containing 6 phosphorylation sites and ribosomal protein-related conserved domains. \u03b1-helices accounted for 70.16\u200a%, \u03b2-turns for 2.42\u200a%, extended strands for 8.87\u200a% and irregular coils for 18.55\u200a% of the secondary structure. The PPIs indicated that L7\/L12 was involved in the constitution of ribosomes and regulating the accuracy of the translation process. Three B-cells, two cytotoxic T lymphocytes and three helper T lymphocyte epitopes were finally screened by comparing multiple databases. L7\/L12 binds to TLR4, BCR, MHC I-TCR and MHC II-TCR complexes and forms stable hydrogen bonds, respectively. L7\/L12, which governs the translation curate of proteins, possesses several potentially advantageous epitopes, laying a theoretical foundation for designing MEVs.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.000786.v3","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T11:17:37Z","timestamp":1728645457000},"source":"Crossref","is-referenced-by-count":3,"title":["Bioinformatics analysis of the antigenic epitopes of L7\/L12 protein in the B- and T-cells active against Brucella melitensis"],"prefix":"10.1099","volume":"6","author":[{"given":"Jingjie","family":"Zhang","sequence":"first","affiliation":[{"name":"Pharmaceutical Laboratory, Inner Mongolia International Mongolian Hospital, Hohhot 010065, PR China"},{"name":"Department of Clinical Laboratory, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010110, PR 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