{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T21:55:12Z","timestamp":1776722112492,"version":"3.51.2"},"reference-count":83,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000008","name":"Health Canada","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000008","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:p>\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    has been involved in transfusion-transmitted fatalities associated with platelet concentrates (PCs) due to its heightened pathogenicity enhanced by genome-encoded virulence and antibiotic resistance genes. This may be facilitated by mobile genetic elements (MGEs) that can cause rearrangements. Several factors contribute to\n                    <jats:italic>S. aureus<\/jats:italic>\n                    virulence, including the type VII secretion system (T7SS), composed of six core genes conserved across\n                    <jats:italic>S. aureus<\/jats:italic>\n                    strains. In this study, we conducted comparative genome analyses of five\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates from PCs (CI\/BAC\/25\/13\u2009\/W, PS\/BAC\/169\/17\u2009\/W and PS\/BAC\/317\/16\u2009\/W were detected during PCs screening with the BACT\/ALERT automated culture system, and ATR-20003 and CBS2016-05 were missed during screening and caused septic transfusion reactions). Multiple alignments of the genomes revealed evidence of rearrangements involving phage Sa3int in PS\/BAC\/169\/17\u2009\/W and PS\/BAC\/317\/16\u2009\/W. While the former had undergone translocation of its immune evasion cluster (IEC), the latter had lost part of the phage, leaving behind the IEC. This observation highlights\n                    <jats:italic>S. aureus<\/jats:italic>\n                    genome plasticity. Unexpectedly, strain CBS2016-05 was found to encode a pseudo-type VII secretion system (T7SS) that had lost five of the conserved core genes (\n                    <jats:italic>esxA<\/jats:italic>\n                    ,\n                    <jats:italic>esaA, essA, esaB<\/jats:italic>\n                    and\n                    <jats:italic>essB<\/jats:italic>\n                    ) and contained a 5\u2032 truncated\n                    <jats:italic>essC<\/jats:italic>\n                    . Since these genes are essential for the function of the T7SS protein transport machinery, which plays a key role in\n                    <jats:italic>S. aureus<\/jats:italic>\n                    virulence, CBS2016-05 probably compensates by recruiting other export mechanisms and\/or alternative virulence factors, such as neu-tralizing immunity proteins. This study unravels genome rearrangements in\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolated from PCs and reports the first\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolate lacking conserved T7SS core genes.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.000820.v4","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T11:18:22Z","timestamp":1726226302000},"source":"Crossref","is-referenced-by-count":2,"title":["Comparative genome analyses of Staphylococcus aureus from platelet concentrates reveal rearrangements involving loss of type VII secretion genes"],"prefix":"10.1099","volume":"6","author":[{"given":"Sylvia","family":"Ighem Chi","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada"},{"name":"Medical Affairs and Innovation, Canadian Blood Services, Ottawa, Ontario, 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