{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:03Z","timestamp":1772156163711,"version":"3.50.1"},"reference-count":26,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001713","name":"European and Developing Countries Clinical Trials Partnership","doi-asserted-by":"crossref","award":["TMA2018CDF-2371"],"award-info":[{"award-number":["TMA2018CDF-2371"]}],"id":[{"id":"10.13039\/501100001713","id-type":"DOI","asserted-by":"crossref"}]},{"name":"MAKRIF","award":["Not given"],"award-info":[{"award-number":["Not given"]}]},{"name":"NURTURE\/NIH","award":["D43TW010132"],"award-info":[{"award-number":["D43TW010132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,19]]},"abstract":"<jats:p>\n                    <jats:bold>Background.<\/jats:bold>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    is part of the ESKAPE (\n                    <jats:italic>Enterococcus faecium<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    spp.) group of multidrug-resistant (MDR) pathogens.\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    is the leading cause of antimicrobial resistance-associated mortality and the second leading cause of nosocomial bloodstream infections (BSIs), globally and in sub-Saharan Africa. Therefore, it was aimed to determine the antibiotic resistance patterns of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolated from blood cultures of patients with features of sepsis at Mulago National Referral Hospital, Uganda.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    The cross-sectional study on patients with features of sepsis utilized\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    =30) isolated from positive blood culture specimens. The antibiotic resistance profile was determined by the Clinical and Laboratory Standards Institute\u2019s Kirby\u2013Bauer disc diffusion method, which was used to classify the isolates as susceptible, intermediate and resistant.\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates that were resistant to third-generation cephalosporins were subjected to extended-spectrum\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -lactamase (ESBL) screening and confirmation using the double-disc synergy test using cefotaxime, ceftazidime, ceftriaxone, cefotaxime\u2013clavulanic acid and ceftazidime\u2013clavulanic acid. The results were analysed for frequencies.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates showed emerging resistance to imipenem at 13% (4 out of 30) followed by amikacin at 17% (5 out of 30). There was intermediate resistance to gentamycin at 60% (18 out of 30). However,\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    showed the highest resistance to piperacillin at 100% (30 out of 30) followed by sulphamethoxazole-trimethoprim and cefepime, both showing a percentage of 97% (29 out of 30). Up to 16 out of 30 (53.3%) of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    were positive for ESBL production, whilst 14 out of 30 (46.7%) were negative.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    There was a high prevalence of antibiotic-resistant\n                    <jats:italic>ESBL<\/jats:italic>\n                    -producing\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates from BSI of patients with features of sepsis in Uganda\u2019s Mulago National Referral Hospital.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.000962.v3","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T09:17:43Z","timestamp":1739956663000},"source":"Crossref","is-referenced-by-count":1,"title":["Tossing the coin of extended-spectrum \u03b2-lactamase: prevalence of extended-spectrum \u03b2-lactamase-producing Klebsiella pneumoniae isolated from patients with sepsis"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9741-5429","authenticated-orcid":false,"given":"Beatrice","family":"Achan","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University Kampala, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1975-1617","authenticated-orcid":false,"given":"Tonny","family":"Luggya","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University Kampala, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1704-6912","authenticated-orcid":false,"given":"Robert Innocent","family":"Ebwongu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University Kampala, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2326-8013","authenticated-orcid":false,"given":"Simon","family":"Sekyanzi","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University Kampala, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2075-0007","authenticated-orcid":false,"given":"Henry","family":"Kajumbula","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University Kampala, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00181-19"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13050431"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00355-19"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2009.03.034"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq005"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02653-2"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-2667(22)"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5520(05)70395-0"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/3511306"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.4314\/ahs.v21i2.4"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0258117"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2019.11.024"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1186\/s40360-015-0013-1"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0286955"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.20471\/acc.2022.61.s1.11"},{"key":"R16","volume-title":"Survey Sampling","author":"Kish","year":"1965"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3665-3669.2004"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-12569-8"},{"key":"R19","article-title":"Trends and spatial distribution of road traffic injuries, uganda, 2012-2023","volume":"9","author":"Kamukama","year":"2024","journal-title":"The Uganda Public Health Bulletin"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.2147\/JMDH.S49075"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlae057"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1007\/s44197-023-00096-2"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-016-2049-8"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.3201\/eid3014.240370"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12111334"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-023-01229-9"}],"container-title":["Access Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/acmi\/10.1099\/acmi.0.000962.v3?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T10:15:48Z","timestamp":1753956948000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/acmi\/10.1099\/acmi.0.000962.v3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,19]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2,19]]}},"URL":"https:\/\/doi.org\/10.1099\/acmi.0.000962.v3","relation":{"has-preprint":[{"id-type":"doi","id":"10.1099\/acmi.0.000962.v1","asserted-by":"subject"},{"id-type":"doi","id":"10.1099\/acmi.0.000962.v2","asserted-by":"subject"},{"id-type":"doi","id":"10.1099\/acmi.0.000962.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1099\/acmi.0.000962.v2","asserted-by":"object"}]},"ISSN":["2516-8290"],"issn-type":[{"value":"2516-8290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,19]]}}}