{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T10:36:14Z","timestamp":1777804574438,"version":"3.51.4"},"reference-count":44,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SOFI 2021","award":["\u201cPReventing the Emergence of untreatable STIs via radical Prevention\u201d (PRESTIP)"],"award-info":[{"award-number":["\u201cPReventing the Emergence of untreatable STIs via radical Prevention\u201d (PRESTIP)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:p>\n                    The resurgence of syphilis has necessitated novel prophylactic strategies, such as the use of doxycycline post-exposure prophylaxis. However, the potential for increased doxycycline use to select for tetracycline resistance represents significant challenges in managing this sexually transmitted infection. This study aims to identify chromosomal mutations associated with tetracycline resistance in\n                    <jats:italic>Spirochaetales<\/jats:italic>\n                    to inform molecular surveillance tools. Whole-genome sequences (WGSs) from the\n                    <jats:italic>Spirochaetales<\/jats:italic>\n                    order, including 4,355 genomes, were analysed for the presence of mutations in 16S rRNA and non-synonymous mutations in the\n                    <jats:italic>rpsC<\/jats:italic>\n                    and\n                    <jats:italic>rpsJ<\/jats:italic>\n                    genes. The study utilized WGS from GenBank\u00ae and sequence data from the PubMLST\n                    <jats:italic>Treponema pallidum<\/jats:italic>\n                    isolate collection. Genetic resistance to tetracycline was detected using a combination of\n                    <jats:sc>blastn<\/jats:sc>\n                    searches and gene\u2013gene analysis. A transition mutation TGA to TGG at positions 965\u2013967 in the 16S rRNA gene was detected in 5.6% of\n                    <jats:italic>Treponema<\/jats:italic>\n                    spp. and 4.0% of\n                    <jats:italic>Spirochaeta<\/jats:italic>\n                    spp. genomes. The\n                    <jats:italic>rpsJ<\/jats:italic>\n                    gene exhibited a V57G\n                    <jats:sc>aa<\/jats:sc>\n                    substitution across a significant subset of\n                    <jats:italic>Treponema<\/jats:italic>\n                    spp. (\n                    <jats:italic>n<\/jats:italic>\n                    =14) and\n                    <jats:italic>Spirochaeta<\/jats:italic>\n                    spp. (\n                    <jats:italic>n<\/jats:italic>\n                    =1). Notably, the V57K substitution was present in\n                    <jats:italic>Spirochaeta<\/jats:italic>\n                    spp. (\n                    <jats:italic>n<\/jats:italic>\n                    =17) and\n                    <jats:italic>Treponema<\/jats:italic>\n                    spp. (\n                    <jats:italic>n<\/jats:italic>\n                    =15). The\n                    <jats:italic>rpsC<\/jats:italic>\n                    gene had the H178Q mutation and was found to be present in the\n                    <jats:italic>Spirochaetales<\/jats:italic>\n                    bacterium (\n                    <jats:italic>n<\/jats:italic>\n                    =4). The identification of putative mutations associated with tetracycline resistance in\n                    <jats:italic>Spirochaetales<\/jats:italic>\n                    provides a foundation for the development of rapid molecular tests. This study underscores the complexity of antibiotic resistance mechanisms and the critical importance of surveillance of genetic resistance determinants in the era of antibiotic prophylaxis for sexually transmitted infection management.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.000963.v4","type":"journal-article","created":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T15:18:48Z","timestamp":1760368728000},"source":"Crossref","is-referenced-by-count":2,"title":["Putative mutations associated with tetracycline resistance detected in Treponema spp.: an analysis of 4,355 Spirochaetales genomes"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8421-5137","authenticated-orcid":false,"given":"Sheeba Santhini","family":"Manoharan-Basil","sequence":"first","affiliation":[{"name":"STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine Antwerp, 2000 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zina","family":"Gestels","sequence":"additional","affiliation":[{"name":"STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine Antwerp, 2000 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sa\u00efd","family":"Abdellati","sequence":"additional","affiliation":[{"name":"Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tessa","family":"de Block","sequence":"additional","affiliation":[{"name":"Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thibaut","family":"Vanbaelen","sequence":"additional","affiliation":[{"name":"STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine 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