{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:29:41Z","timestamp":1789187381073,"version":"build-2803163510"},"reference-count":39,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100024597","name":"Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation, Thailand","doi-asserted-by":"crossref","award":["RGNS 65-156"],"award-info":[{"award-number":["RGNS 65-156"]}],"id":[{"id":"10.13039\/501100024597","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"crossref","award":["MR\/W030578\/1"],"award-info":[{"award-number":["MR\/W030578\/1"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,1]]},"abstract":"<jats:p>\n                    <jats:bold>Aim.<\/jats:bold>\n                    Antimicrobial resistance poses a critical global health threat, driven by the dissemination of resistance genes via mobile genetic elements (MGEs). This study aims to enhance the detection of MGE insertions in multidrug-resistant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    by derivatizing the pBACpAK entrapment vector.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods and results.<\/jats:bold>\n                    Three derivatives were constructed with additional nucleotides upstream of the\n                    <jats:italic>cI<\/jats:italic>\n                    repressor gene, based on conserved regions identified from GenBank sequences containing known IS\n                    <jats:italic>26<\/jats:italic>\n                    and IS\n                    <jats:italic>1<\/jats:italic>\n                    insertions. Using colony PCR, intracellular transposition screening was performed on 194 tetracycline-resistant colonies from four\n                    <jats:italic>E. coli<\/jats:italic>\n                    ESI123 strains carrying different pBACpAK constructs. The derivatives showed increased MGE capture rates (10.7\u201373.1\u200a%) compared to the WT vector (3.75%), identifying multiple MGEs, including the novel composite transposon Tn\n                    <jats:italic>7824<\/jats:italic>\n                    . Tn\n                    <jats:italic>7824<\/jats:italic>\n                    harbours the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-181<\/jats:sub>\n                    carbapenem resistance gene and the\n                    <jats:italic>qnrS1<\/jats:italic>\n                    quinolone resistance gene, highlighting the clinical relevance of these findings. Long-read sequencing of transposants confirmed the accuracy of MGE identification and structural characterization, which also revealed chromosomal integration events of the pBACpAK derivatives mediated by flanking insertion sequences.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions.<\/jats:bold>\n                    The modifications introduced in the pBACpAK derivatives could increase the detection of transposition events by alleviating spatial constraints, allowing for more robust MGE detection.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.001013.v3","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T08:17:20Z","timestamp":1747988240000},"source":"Crossref","is-referenced-by-count":2,"title":["Derivatization of pBACpAK entrapment vectors for enhanced mobile genetic element transposition detection in multidrug-resistant Escherichia 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