{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:24:49Z","timestamp":1777983889657,"version":"3.51.4"},"reference-count":34,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,1]]},"abstract":"\n Background.<\/jats:bold>\n The increasing prevalence of tigecycline and carbapenem-resistant\n Klebsiella pneumoniae<\/jats:italic>\n (CRKP) poses a serious challenge, especially in resource-limited settings. Its ability to exchange resistance genes with other bacteria accelerates the spread of multidrug resistance. While carbapenems and tetracyclines have been used effectively against\n K. pneumoniae<\/jats:italic>\n , resistance to these agents is now rising globally, narrowing available treatment options.\n <\/jats:p>\n \n Objective.<\/jats:bold>\n The study aimed to determine the phenotypic and genotypic prevalence of carbapenem and tetracycline resistance in\n K. pneumoniae<\/jats:italic>\n isolates along with the transferability pattern of carbapenem and tetracycline resistance genes in these isolates.\n <\/jats:p>\n \n Methodology.<\/jats:bold>\n Clinical isolates from pus and respiratory samples were identified using biochemical tests and MALDI-TOF MS. Antimicrobial susceptibility test was performed by the Kirby\u2013Bauer disc diffusion method, and MICs were determined by the broth microdilution test method. PCR was performed to detect carbapenemase (\n bla<\/jats:italic>\n NDM<\/jats:sub>\n ,\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n and\n bla<\/jats:italic>\n KPC<\/jats:sub>\n ) and tetracycline resistance genes [\n tet(A)<\/jats:italic>\n ,\n tet(B)<\/jats:italic>\n ,\n tet(K)<\/jats:italic>\n ,\n tet(M)<\/jats:italic>\n and\n tet(S)<\/jats:italic>\n ], followed by Sanger sequencing for validation. Conjugation assays assessed gene transferability.\n <\/jats:p>\n \n Results.<\/jats:bold>\n Out of 152 CRKP isolates, 20.4% (31 out of 152) were found to be resistant to tigecycline. All tigecycline-resistant isolates exhibited complete resistance (31 out of 31; 100%) to ceftazidime, ciprofloxacin and omadacycline. Additionally, resistance to amikacin and cefoperazone-sulbactam was observed in 87.1% (27 out of 31) and 77.4% (24 out of 31) of the isolates. Resistance to minocycline and colistin was detected in 51.6% (16 out of 31) and 29.0% (9 out of 31) of the isolates, respectively.\n <\/jats:p>\n \n PCR analysis revealed that 51.6% (16 out of 31) of the isolates carried the\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n gene, and 29.0% (9 out of 31) carried the\n bla<\/jats:italic>\n NDM<\/jats:sub>\n gene. None of the isolates harboured the\n bla<\/jats:italic>\n KPC<\/jats:sub>\n gene. With respect to tetracycline resistance determinants, the\n tet(A)<\/jats:italic>\n gene was detected in 12.9% (4 out of 31) of the isolates, and the\n tet(B<\/jats:italic>\n ) gene in 3.2% (1 out of 31), while\n tet(K)<\/jats:italic>\n ,\n tet(M)<\/jats:italic>\n ,\n tet(S)<\/jats:italic>\n and\n bla<\/jats:italic>\n KPC<\/jats:sub>\n were not detected in any isolate.\n <\/jats:p>\n \n Conjugation assays demonstrated that plasmids carrying\n bla<\/jats:italic>\n NDM<\/jats:sub>\n and\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n were transferable to a recipient strain, indicating their potential for horizontal gene transfer. In contrast, plasmids harbouring\n tet(A)<\/jats:italic>\n and\n tet(B)<\/jats:italic>\n genes were not transferable under the experimental conditions.\n <\/jats:p>\n \n Conclusion.<\/jats:bold>\n Tigecycline-resistant\n K. pneumoniae<\/jats:italic>\n isolates showed high multidrug resistance, with transferable\n bla<\/jats:italic>\n NDM<\/jats:sub>\n and\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n genes. In contrast, chromosome and plasmid-borne tetracycline resistance genes\n tet(A)<\/jats:italic>\n and\n tet(B)<\/jats:italic>\n were non-transferable, indicating limited horizontal spread.\n <\/jats:p>","DOI":"10.1099\/acmi.0.001017.v4","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T17:16:46Z","timestamp":1773335806000},"source":"Crossref","is-referenced-by-count":0,"title":["Molecular characterization and transmission pattern of tetracycline resistance determinants in tigecycline and carbapenem resistant Klebsiella pneumoniae isolates at a tertiary care hospital in India"],"prefix":"10.1099","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1543-4499","authenticated-orcid":false,"given":"Jyoti","family":"Chaudhary","sequence":"first","affiliation":[{"name":"Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, C Block, 2nd Floor, SGPGIMS Lucknow, UP \u2013 226014, 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