{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:28:49Z","timestamp":1787243329504,"version":"build-2736575974"},"reference-count":22,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"crossref","award":["CRS115_205933"],"award-info":[{"award-number":["CRS115_205933"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:p>Monkeypox virus (MPXV) is a zoonotic pathogen that has recently caused outbreaks in non-endemic areas. Wastewater-based surveillance is independent of individual testing and offers substantial potential for monitoring population-level disease dynamics of MPXV at the clade level, as clades differ in transmissibility and mortality rate. Here, we report the validation of a four-plex digital PCR assay to detect and quantify the major clades and subclades of MPXV. The assay demonstrated strong linearity, analytical sensitivity (limits of detection ranged from 6.7 to 9.6 copies per reaction across targets) and specificity in distinguishing and quantifying MPXV by clade, which is advantageous for both clinical and wastewater applications.<\/jats:p>","DOI":"10.1099\/acmi.0.001041.v3","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:15:49Z","timestamp":1787242549000},"source":"Crossref","is-referenced-by-count":0,"title":["Clade-specific multiplex digital PCR assay for monkeypox virus detection in spike-in wastewater samples"],"prefix":"10.1099","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5381-3132","authenticated-orcid":false,"given":"Melissa","family":"Pitton","sequence":"first","affiliation":[{"name":"Eawag, Swiss Federal Institute of Aquatic Science and Technology, D\u00fcbendorf, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6554-0954","authenticated-orcid":false,"given":"Charles","family":"Gan","sequence":"additional","affiliation":[{"name":"Eawag, Swiss Federal Institute of Aquatic Science and Technology, D\u00fcbendorf, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9776-185X","authenticated-orcid":false,"given":"Patrick","family":"Schmidhalter","sequence":"additional","affiliation":[{"name":"Eawag, Swiss Federal Institute of Aquatic Science and Technology, D\u00fcbendorf, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9950-2286","authenticated-orcid":false,"given":"Christoph","family":"Ort","sequence":"additional","affiliation":[{"name":"Eawag, Swiss Federal Institute of Aquatic Science and Technology, D\u00fcbendorf, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1000-0306","authenticated-orcid":false,"given":"Timothy R.","family":"Julian","sequence":"additional","affiliation":[{"name":"University of Basel, 4055 Basel, Switzerland"},{"name":"Swiss Tropical and Public Health Institute, 4123 Allschwil, Switzerland"},{"name":"Eawag, Swiss Federal Institute of Aquatic Science and Technology, D\u00fcbendorf, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.07.012"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-09537-0"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2023.105421"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1093\/jtm\/taad111"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.57187\/s.3706"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-024-09603-5"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.lana.2023.100639"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.158265"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.159816"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2023.119621"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2213882"},{"key":"R12","article-title":"Considerations for wastewater and environmental surveillance for monkeypox virus","year":"2024"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2024.29.32.2400486"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2015.12.003"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.29"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/s44221-025-00503-x"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.57187\/s.3503"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153317"},{"key":"R19","article-title":"Mpox (Monkeypox)"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.173108"},{"key":"R21","article-title":"Mpox: Multi-country External Situation Report n.43","year":"2024"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00018-24"}],"container-title":["Access Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/acmi\/10.1099\/acmi.0.001041.v3?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:15:50Z","timestamp":1787242550000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/acmi\/10.1099\/acmi.0.001041.v3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,1]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,1]]}},"URL":"https:\/\/doi.org\/10.1099\/acmi.0.001041.v3","relation":{"has-preprint":[{"id-type":"doi","id":"10.1099\/acmi.0.001041.v1","asserted-by":"subject"},{"id-type":"doi","id":"10.1099\/acmi.0.001041.v2","asserted-by":"subject"},{"id-type":"doi","id":"10.1099\/acmi.0.001041.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1099\/acmi.0.001041.v2","asserted-by":"object"}]},"ISSN":["2516-8290"],"issn-type":[{"value":"2516-8290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,1]]}}}