{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T07:26:11Z","timestamp":1781853971995,"version":"3.54.5"},"reference-count":49,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Precision System Science"},{"name":"Precision System Science"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:p>\n                    Rapid and accurate detection of vancomycin-resistant enterococci (VREs) can aid in the early application of appropriate antimicrobial treatment and the implementation of infection prevention measures. The VIASURE real-time PCR assay (Certest Biotec) is a multiplex nucleic acid-based\n                    <jats:italic>in vitro<\/jats:italic>\n                    diagnostic test intended for the detection of\n                    <jats:italic>vanA<\/jats:italic>\n                    and\n                    <jats:italic>vanB<\/jats:italic>\n                    , and geneLEAD VIII (Precision System Science) is a customizable, fully automated molecular detection platform. We evaluated the performance of the VIASURE assay on the geneLEAD VIII platform against 200 clinical enterococcal isolates consisting of 151 VREs and 49 vancomycin-susceptible enterococci collected in Japan, primarily in the Kinki region, and compared it to that of the in-house reference multiplex PCR assay. The performance of the VIASURE assay for the detection of\n                    <jats:italic>vanA<\/jats:italic>\n                    and\n                    <jats:italic>vanB<\/jats:italic>\n                    was comparable to that of the reference multiplex PCR assay, with both a sensitivity and specificity of 100%. Compared with the reference PCR assay, the VIASURE assay reduced the turn-around time by ~3\u2009h 40\u2009min (5\u2009h 34\u2009min vs. 1\u2009h 54\u2009min) and the hands-on time by 46\u2009min per four samples. Fully automated molecular detection of\n                    <jats:italic>vanA<\/jats:italic>\n                    and\n                    <jats:italic>vanB<\/jats:italic>\n                    using the VIASURE assay and geneLEAD VIII for bacterial isolates can enable fast and reliable testing while reducing labour and is a promising tool for clinical laboratories and nosocomial infection control.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.001044.v4","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T14:15:24Z","timestamp":1754576124000},"source":"Crossref","is-referenced-by-count":2,"title":["Automated molecular detection of the vancomycin resistance genes vanA and vanB using the geneLEAD VIII platform"],"prefix":"10.1099","volume":"7","author":[{"given":"Keiichiro","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Infection Control and Prevention, Kyoto University Hospital, Kyoto, Japan"},{"name":"Department of 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