{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:10Z","timestamp":1772156170868,"version":"3.50.1"},"reference-count":41,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"USDA National Institute of Food and Agriculture","doi-asserted-by":"crossref","award":["Hatch project number 1004830 and from USDA ARS Project No. 8072-42000-077"],"award-info":[{"award-number":["Hatch project number 1004830 and from USDA ARS Project No. 8072-42000-077"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>\n                    Bioluminescent bioreporters are widely used across various scientific disciplines due to the well-characterized bacterial bioluminescence mechanism. However, solvent-induced membrane perturbations may confound the use of bioreporters in assessing cellular toxicity from environmental contaminants. This study investigated the solvent effect, wherein membrane damage increases intracellular availability of bioluminescent reaction precursors, increasing the light produced. A new online\n                    <jats:italic>in situ<\/jats:italic>\n                    monitoring system was also tested with multiple bioluminescent reporters, including a newly constructed\n                    <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n                    M3A strain, exposed to toluene, trichloroethylene, acetone, phenol and creosote derived from beechwood tar. Additional tests included the introduction of carbon nanotubes, fullerene and fullerenol. A solvent effect was confirmed by the detection of increased bioluminescent signal and the occurrence of fatty acid release (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05). Phenol (25 p.p.m.), a benchmark for bactericidal activity, demonstrated luminescence enhancement via the solvent effect. Membrane toxicity assays showed that\n                    <jats:italic>P. fluorescens<\/jats:italic>\n                    M3A responded sensitively to sublethal and lethal membrane disruptions, whereas\n                    <jats:italic>Vibrio fischeri<\/jats:italic>\n                    MJ1 did not exhibit a solvent effect, and its luminescence changes were not correlated with viability (\n                    <jats:italic>P<\/jats:italic>\n                    &gt;0.05). These results indicate that\n                    <jats:italic>P. fluorescens<\/jats:italic>\n                    M3A is a sensitive biosensor for detecting environmental contaminants and identifying both lethal and sublethal membrane perturbations. The findings underscore essential considerations when utilizing bacterial bioluminescence as a proxy for gene expression or cellular physiology.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.001096.v3","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T15:17:52Z","timestamp":1767885472000},"source":"Crossref","is-referenced-by-count":0,"title":["Application of the solvent effect on bioluminescent reporter bacteria as a real-time membrane toxicity assay"],"prefix":"10.1099","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1980-2105","authenticated-orcid":false,"given":"Phillip R.","family":"Myer","sequence":"first","affiliation":[{"name":"Department of Animal Science, University of Tennessee, Knoxville, TN, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald F.","family":"Turco","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Purdue University, West Lafayette, IN, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce M.","family":"Applegate","sequence":"additional","affiliation":[{"name":"Department of Food Science, Purdue University, West Lafayette, IN, 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