{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:02:56Z","timestamp":1790114576730,"version":"4.0.1"},"reference-count":67,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>\n                    This secondary\n                    <jats:italic>in silico<\/jats:italic>\n                    study analysed 36\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    draft genome assemblies from Zambia to characterize the virulence gene profiles of extraintestinal pathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    (ExPEC) and to relate them to mobilome proxies, namely plasmid replicons and prophage content. All assemblies met plausibility thresholds for\n                    <jats:italic>E. coli<\/jats:italic>\n                    (1\u201317 contigs; 4.85\u20135.77\u2009Mb; G+C 50.47\u201350.96 mol%), and species identity was confirmed by average nucleotide identity to the\n                    <jats:italic>E. coli<\/jats:italic>\n                    type strain (96.6\u201398.9%). Using the Achtman seven-locus MLST scheme applied to the reads, 35 of 36 genomes were assigned a sequence type (ST), and 1 remained untypeable at a single locus. The most frequent type was ST69 (9 out of 36) rather than the globally dominant ST131 (6 out of 36), followed by ST405 (5 out of 36) and ST617 (5 out of 36). Phylogroup D was most frequent (12 out of 36), followed by the commensal-associated phylogroup A (8 out of 36) and the pandemic phylogroup B2 (6 out of 36). Classical intestinal-pathotype hallmarks (\n                    <jats:italic>stx1<\/jats:italic>\n                    ,\n                    <jats:italic>stx2<\/jats:italic>\n                    ,\n                    <jats:italic>eae<\/jats:italic>\n                    ,\n                    <jats:italic>bfpA<\/jats:italic>\n                    and\n                    <jats:italic>ipaH<\/jats:italic>\n                    ) were not detected. In contrast, ExPEC-associated markers were common, including\n                    <jats:italic>iutA<\/jats:italic>\n                    (27 out of 36),\n                    <jats:italic>iss2<\/jats:italic>\n                    (26 out of 36),\n                    <jats:italic>fyuA<\/jats:italic>\n                    (24 out of 36),\n                    <jats:italic>kpsM<\/jats:italic>\n                    (23 out of 36) and\n                    <jats:italic>ompT<\/jats:italic>\n                    (18 out of 36), with a high within-genome burden (median ~193 loci; range 173\u2013251). Gene-based rules classified 19 of 36 genomes as ExPEC and 2 as enteroaggregative\n                    <jats:italic>E. coli<\/jats:italic>\n                    , and source-informed analysis identified uropathogenic, neonatal meningitis-associated and sepsis-associated subpathotypes. IncF-family replicons predominated,\n                    <jats:italic>sitABCD<\/jats:italic>\n                    occurred in both chromosomal and IncF-plasmid contexts, and\n                    <jats:italic>iss2<\/jats:italic>\n                    was recurrently prophage-associated. These findings indicate that extraintestinal virulence in this population is distributed and modular rather than confined to ST131. This virulence is carried across diverse lineages and phylogroups, including classically commensal backgrounds, and is mobilized by plasmids and prophages. Ultimately, these data provide a baseline for the genomic surveillance of virulent\n                    <jats:italic>E. coli<\/jats:italic>\n                    in Zambia.\n                  <\/jats:p>","DOI":"10.1099\/acmi.0.001165.v3","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:16:20Z","timestamp":1790111780000},"source":"Crossref","is-referenced-by-count":0,"title":["Virulence gene profiles of extraintestinal pathogenic Escherichia coli (ExPEC) from Zambia: a secondary in silico study"],"prefix":"10.1099","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7251-2232","authenticated-orcid":false,"given":"Frank Chilombolwa","family":"Nyondo","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Ridgeway Campus, University of Zambia, School of Health Sciences, Lusaka, 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