{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:46:14Z","timestamp":1792111574295,"version":"4.4.0"},"reference-count":31,"publisher":"Microbiology Society","issue":"Pt_4","funder":[{"name":"Ministry of Economy, Trade and Industry (METI) of Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:p>A methanogenic archaeon, strain HC-2<jats:sup>T<\/jats:sup>, was isolated from a deep diatomaceous shale formation. The strain grew on methanol, monomethylamine, dimethylamine, trimethylamine and dimethylsulphide, but not on acetate, H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, formate, 2-propanol, 2-butanol or cyclopentanol. Cells were Gram-stain-negative, non-motile, and coccus-like, 0.9\u20131.4 \u00b5m in diameter, and occurred singly, in pairs, or as aggregates. The strain grew at 10\u201340 \u00b0C (optimum 35 \u00b0C), pH 5.9\u20137.4 (optimum pH 6.6\u20136.8) and in 0\u20130.6 M NaCl (optimum 0.1\u20130.2 M). The genomic DNA G+C content was 41.5 mol% and the 16S rRNA gene sequence was closely related to those of <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.233\" xlink:type=\"simple\">\n                        <jats:italic>Methanosarcina lacustris<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content> DSM 13486<jats:sup>T<\/jats:sup> (99.1\u200a%) and <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.237\" xlink:type=\"simple\">\n                        <jats:italic>Methanosarcina siciliae<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content> DSM 3028<jats:sup>T<\/jats:sup> (98.3\u200a%). Values for DNA\u2013DNA hybridization with these strains were less than 30\u200a%. The phenotypic and phylogenetic features of HC-2<jats:sup>T<\/jats:sup> indicate that it represents a novel species of the genus <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.228\" xlink:type=\"simple\">\n                        <jats:italic>Methanosarcina<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content>, for which the name <jats:italic>Methanosarcina subterranea<\/jats:italic> sp. nov. is proposed. The type strain is HC-2<jats:sup>T<\/jats:sup> (\u200a=\u200aDSM 22503<jats:sup>T<\/jats:sup>\u200a=\u200aJCM 15540<jats:sup>T<\/jats:sup>\u200a=\u200aNBRC 102578<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.000072","type":"journal-article","created":{"date-parts":[[2015,1,21]],"date-time":"2015-01-21T04:58:11Z","timestamp":1421816291000},"page":"1167-1171","source":"Crossref","is-referenced-by-count":39,"title":["Methanosarcina subterranea sp. nov., a methanogenic archaeon isolated from a deep subsurface diatomaceous shale formation"],"prefix":"10.1099","volume":"65","author":[{"given":"Satoru","family":"Shimizu","sequence":"first","affiliation":[{"name":"Horonobe Research Institute for the Subsurface Environment, Northern Advancement Center for Science and Technology, Horonobe-cho, Teshio-gun, Hokkaido 098-3221, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Ueno","sequence":"additional","affiliation":[{"name":"Horonobe Research Institute for the Subsurface Environment, Northern Advancement Center for Science and Technology, Horonobe-cho, Teshio-gun, Hokkaido 098-3221, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Naganuma","sequence":"additional","affiliation":[{"name":"Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Horonobe Research Institute for the Subsurface Environment, Northern Advancement Center for Science and Technology, Horonobe-cho, Teshio-gun, Hokkaido 098-3221, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1099\/00207713-38-2-212","article-title":"Proposal of minimal standards for describing new taxa of methanogenic 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