{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:21:11Z","timestamp":1791418871828,"version":"4.3.3"},"reference-count":24,"publisher":"Microbiology Society","issue":"Pt_7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,1]]},"abstract":"<jats:p>Nine <jats:italic>Burkholderia cepacia<\/jats:italic> complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of <jats:italic>Burkholderia pseudomallei<\/jats:italic>, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9\u200a%, respectively) and towards established Bcc species (4.0 and 3.9\u200a%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and <jats:italic>recA<\/jats:italic> gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of <jats:italic>recA<\/jats:italic> gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of <jats:italic>N<\/jats:italic>-acetylglucosamine. The names <jats:italic>Burkholderia stagnalis<\/jats:italic> sp. nov. with type strain LMG 28156<jats:sup>T<\/jats:sup> (\u2009=\u2009CCUG 65686<jats:sup>T<\/jats:sup>) and <jats:italic>Burkholderia territorii<\/jats:italic> sp. nov. with type strain LMG 28158<jats:sup>T<\/jats:sup> (\u2009=\u2009CCUG 65687<jats:sup>T<\/jats:sup>) are proposed for Bcc B and Bcc L bacteria, respectively.<\/jats:p>","DOI":"10.1099\/ijs.0.000251","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T01:12:29Z","timestamp":1429060349000},"page":"2265-2271","source":"Crossref","is-referenced-by-count":138,"title":["Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources"],"prefix":"10.1099","volume":"65","author":[{"given":"Birgit","family":"De Smet","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium"},{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Mayo","sequence":"additional","affiliation":[{"name":"Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Peeters","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E. 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Kidd","sequence":"additional","affiliation":[{"name":"Queensland Children's Medical Research Institute, University of Queensland, Brisbane, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjam","family":"Kaestli","sequence":"additional","affiliation":[{"name":"Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Ginther","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ, 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