{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:56Z","timestamp":1791942416469,"version":"4.3.4"},"reference-count":31,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:p>A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1<jats:sup>T<\/jats:sup>) was isolated recently from a breast implant infection of a 71-year-old female patient with clinical signs of brucellosis. Affiliation of strain BO1<jats:sup>T<\/jats:sup> to the genus <jats:italic>Brucella<\/jats:italic> was confirmed by means of polyamine pattern, polar lipid profile, fatty acid profile, quinone system, DNA\u2013DNA hybridization studies and by insertion sequence <jats:italic>711<\/jats:italic> (IS<jats:italic>711<\/jats:italic>)-specific PCR. Strain BO1<jats:sup>T<\/jats:sup> harboured four to five copies of the <jats:italic>Brucella<\/jats:italic>-specific insertion element IS<jats:italic>711<\/jats:italic>, displaying a unique banding pattern, and exhibited a unique 16S rRNA gene sequence and also grouped separately in multilocus sequence typing analysis. Strain BO1<jats:sup>T<\/jats:sup> reacted with <jats:italic>Brucella<\/jats:italic> M-monospecific antiserum. Incomplete lysis was detected with bacteriophages Tb (Tbilisi), F1 and F25. Biochemical profiling revealed a high degree of enzymic activity and metabolic capabilities. In multilocus VNTR (variable-number tandem-repeat) analysis, strain BO1<jats:sup>T<\/jats:sup> showed a very distinctive profile and clustered with the other \u2018exotic\u2019 <jats:italic>Brucella<\/jats:italic> strains, including strains isolated from marine mammals, and <jats:italic>Brucella microti<\/jats:italic>, <jats:italic>Brucella suis<\/jats:italic> biovar 5 and <jats:italic>Brucella neotomae<\/jats:italic>. Comparative <jats:italic>omp2a<\/jats:italic> and <jats:italic>omp2b<\/jats:italic> gene sequence analysis revealed the most divergent <jats:italic>omp2<\/jats:italic> sequences identified to date for a <jats:italic>Brucella<\/jats:italic> strain. The <jats:italic>recA<\/jats:italic> gene sequence of strain BO1<jats:sup>T<\/jats:sup> differed in seven nucleotides from the <jats:italic>Brucella recA<\/jats:italic> consensus sequence. Using the <jats:italic>Brucella<\/jats:italic> species-specific multiplex PCR assay, strain BO1<jats:sup>T<\/jats:sup> displayed a unique banding pattern not observed in other <jats:italic>Brucella<\/jats:italic> species. From the phenotypic and molecular analysis it became evident that strain BO1<jats:sup>T<\/jats:sup> was clearly different from all other <jats:italic>Brucella<\/jats:italic> species, and therefore represents a novel species within the genus <jats:italic>Brucella<\/jats:italic>. Because of its unexpected isolation, the name <jats:italic>Brucella inopinata<\/jats:italic> with the type strain BO1<jats:sup>T<\/jats:sup> (=BCCN 09-01<jats:sup>T<\/jats:sup>=CPAM 6436<jats:sup>T<\/jats:sup>) is proposed.<\/jats:p>","DOI":"10.1099\/ijs.0.011148-0","type":"journal-article","created":{"date-parts":[[2009,11,3]],"date-time":"2009-11-03T22:56:16Z","timestamp":1257288976000},"page":"801-808","source":"Crossref","is-referenced-by-count":258,"title":["Brucella inopinata sp. nov., isolated from a breast implant infection"],"prefix":"10.1099","volume":"60","author":[{"given":"Holger C.","family":"Scholz","sequence":"first","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"N\u00f6ckler","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, 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