{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:48:36Z","timestamp":1787010516751,"version":"build-2736575974"},"reference-count":38,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"\n Two strains of Gram-positive cocci were isolated from viscera of common voles (\n Microtus arvalis<\/jats:italic>\n Pallas) with generalized\n Brucella microti<\/jats:italic>\n infection in the Czech Republic. Biochemical features and phylogenetic analysis based on 16S rRNA gene sequences showed that the strains are representatives of the genus\n Staphylococcus<\/jats:italic>\n and assigned\n Staphylococcus muscae<\/jats:italic>\n as the nearest relative. A detailed characterization done by ribotyping,\n rpoB<\/jats:italic>\n and\n hsp60<\/jats:italic>\n gene sequencing, whole-cell protein analysis and rep-PCR using the (GTG)\n 5<\/jats:sub>\n primer differentiated the two strains from all described staphylococci. DNA\u2013DNA hybridization with the type strain of\n S. muscae<\/jats:italic>\n demonstrated that the two strains should be considered as members of a novel species (26.8\u200a% reassociation). The two analysed strains were found to be coagulase-negative, novobiocin-susceptible, oxidase-negative cultures, phenotypically close to one another, but showing differences in ribotype profiles. The major fatty acids were iso-C\n 15\u200a:\u200a0<\/jats:sub>\n , iso-C\n 17\u200a:\u200a0<\/jats:sub>\n , anteiso-C\n 15\u200a:\u200a0<\/jats:sub>\n , C\n 18\u200a:\u200a2<\/jats:sub>\n \u03c9<\/jats:italic>\n 6,9\n c<\/jats:italic>\n \/anteiso-C\n 18\u200a:\u200a0<\/jats:sub>\n , C\n 18\u200a:\u200a0<\/jats:sub>\n and C\n 18\u200a:\u200a1<\/jats:sub>\n \u03c9<\/jats:italic>\n 9\n c<\/jats:italic>\n . MK-7 was the predominant isoprenoid quinone, with minor amounts of MK-6 and MK-8. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylglycerol and several unknown lipids. These results proved that the two isolates represent a novel staphylococcal species. The name proposed for this novel taxon is\n Staphylococcus microti<\/jats:italic>\n sp. nov.; the type strain is 4005-LJ(m)\n T<\/jats:sup>\n (=CCM 4903\n T<\/jats:sup>\n =CCUG 55861\n T<\/jats:sup>\n =DSM 22147\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijs.0.011429-0","type":"journal-article","created":{"date-parts":[[2009,11,3]],"date-time":"2009-11-03T17:56:16Z","timestamp":1257270976000},"page":"566-573","source":"Crossref","is-referenced-by-count":26,"title":["Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)"],"prefix":"10.1099","volume":"60","author":[{"given":"Dana","family":"Nov\u00e1kov\u00e1","sequence":"first","affiliation":[{"name":"Masaryk University, Institute of Experimental Biology, Czech Collection of Microorganisms, Tvrd\u00e9ho 14, CZ-602 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Pant\u016f\u010dek","sequence":"additional","affiliation":[{"name":"Masaryk University, Institute of Experimental Biology, Department of Genetics and Molecular Biology, Kotl\u00e1\u0159sk\u00e12, CZ-611 37 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zden\u011bk","family":"Hub\u00e1lek","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology, Academy of Sciences, Kv\u011btn\u00e1 8, CZ-603 65 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enevold","family":"Falsen","sequence":"additional","affiliation":[{"name":"Culture Collection, University of G\u00f6teborg, Department of Clinical Bacteriology, Guldhedsgatan 10, SE-413 46 G\u00f6teborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-J\u00fcrgen","family":"Busse","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Bakteriologie, Mykologie und Hygiene, Veterin\u00e4rmedizinische Universit\u00e4t, Veterin\u00e4rplatz 1, A-1210 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Schumann","sequence":"additional","affiliation":[{"name":"Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo","family":"Sedl\u00e1\u010dek","sequence":"additional","affiliation":[{"name":"Masaryk University, Institute of Experimental Biology, Czech Collection of Microorganisms, Tvrd\u00e9ho 14, CZ-602 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0168-1656(96)01376-4","article-title":"Classification of bacteria isolated from a medieval wall painting","volume":"47","author":"Altenburger","year":"1996","journal-title":"J Biotechnol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1099\/00207713-50-2-689","article-title":"The value of rRNA gene restriction site polymorphism analysis for delineating taxa in the genus Staphylococcus","volume":"50","author":"Chesneau","year":"2000","journal-title":"Int J Syst Evol Microbiol"},{"key":"R3","author":"Devriese","year":"1978"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1099\/ijs.0.63413-0","article-title":"Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals","volume":"55","author":"Devriese","year":"2005","journal-title":"Int J Syst Evol Microbiol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1128\/JCM.26.9.1745-1753.1988","article-title":"Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids","volume":"26","author":"Eerola","year":"1988","journal-title":"J Clin Microbiol"},{"key":"R6","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1099\/00207713-47-3-724","article-title":"Staphylococcus lutrae sp. nov., a new coagulase-positive species isolated from otters","volume":"47","author":"Foster","year":"1997","journal-title":"Int J Syst Bacteriol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1099\/00207713-38-2-168","article-title":"Staphylococcus lugdunensis sp. nov. and Staphylococcus schleiferi sp. nov., two species from human clinical specimens","volume":"38","author":"Freney","year":"1988","journal-title":"Int J Syst Bacteriol"},{"key":"R8","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1099\/00207713-49-2-489","article-title":"Recommended minimal standards for description of new staphylococcal species. 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