{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:10:49Z","timestamp":1790507449139,"version":"4.1.0"},"reference-count":25,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:p>Two bacterial strains, Fuji 18-1<jats:sup>T<\/jats:sup>and Fuji 23-1<jats:sup>T<\/jats:sup>, were isolated from the blood of the small Japanese field mouse (<jats:italic>Apodemus argenteus<\/jats:italic>) and the large Japanese field mouse (<jats:italic>Apodemus speciosus<\/jats:italic>), respectively, specimens of which were captured in the forest of Mount Fuji, Japan. Phenotypic characterization (growth conditions, incubation periods, biochemical properties and cell morphologies), DNA G+C contents (40.1\u2005mol% for strain Fuji 18-1<jats:sup>T<\/jats:sup>and 40.4\u2005mol% for strain Fuji 23-1<jats:sup>T<\/jats:sup>) and sequence analyses of the 16S rRNA genes indicated that both strains were members of the genus<jats:italic>Bartonella<\/jats:italic>. Using<jats:italic>rpoB<\/jats:italic>and<jats:italic>gltA<\/jats:italic>sequencing analysis, the highest sequence similarities between strains Fuji 18-1<jats:sup>T<\/jats:sup>, Fuji 23-1<jats:sup>T<\/jats:sup>and other recognized species of the genus<jats:italic>Bartonella<\/jats:italic>showed values considerably lower than 91.4\u200a% and 89.9\u200a% in the<jats:italic>rpoB<\/jats:italic>gene and 89.1\u200a% and 90.4\u200a% in the<jats:italic>gltA<\/jats:italic>gene, respectively. It is known that similarities of 95.4\u200a% for the<jats:italic>rpoB<\/jats:italic>gene and 96.0\u200a% for the<jats:italic>gltA<\/jats:italic>gene can be applied as cut-off values for the designation of novel species of the genus<jats:italic>Bartonella<\/jats:italic>. In a phylogenetic tree based on the merged set of concatenated sequences of seven loci [16S rRNA,<jats:italic>ftsZ<\/jats:italic>,<jats:italic>gltA<\/jats:italic>,<jats:italic>groEL<\/jats:italic>,<jats:italic>ribC<\/jats:italic>and<jats:italic>rpoB<\/jats:italic>genes and the intergenic spacer region (ITS)], strains Fuji 18-1<jats:sup>T<\/jats:sup>and Fuji 23-1<jats:sup>T<\/jats:sup>formed a distinct clade from other recognized species of the genus<jats:italic>Bartonella<\/jats:italic>. These data support the classification of strains Fuji 18-1<jats:sup>T<\/jats:sup>and Fuji 23-1<jats:sup>T<\/jats:sup>as novel species of the genus<jats:italic>Bartonella<\/jats:italic>. The names<jats:italic>Bartonella japonica<\/jats:italic>sp. nov. and<jats:italic>Bartonella silvatica<\/jats:italic>sp. nov. are proposed for these novel species. The type strains of<jats:italic>Bartonella japonica<\/jats:italic>sp. nov. and<jats:italic>Bartonella silvatica<\/jats:italic>sp. nov. are Fuji 18-1<jats:sup>T<\/jats:sup>(=JCM 15567<jats:sup>T<\/jats:sup>=CIP 109861<jats:sup>T<\/jats:sup>) and Fuji 23-1<jats:sup>T<\/jats:sup>(=JCM 15566<jats:sup>T<\/jats:sup>=CIP 109862<jats:sup>T<\/jats:sup>), respectively.<\/jats:p>","DOI":"10.1099\/ijs.0.011528-0","type":"journal-article","created":{"date-parts":[[2009,11,3]],"date-time":"2009-11-03T22:56:16Z","timestamp":1257288976000},"page":"759-763","source":"Crossref","is-referenced-by-count":41,"title":["Bartonella japonica sp. nov. and Bartonella silvatica sp. nov., isolated from Apodemus mice"],"prefix":"10.1099","volume":"60","author":[{"given":"Kai","family":"Inoue","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidenori","family":"Kabeya","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatsumi","family":"Shiratori","sequence":"additional","affiliation":[{"name":"Life Science Research Center, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Ueda","sequence":"additional","affiliation":[{"name":"Life Science Research Center, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Y.","family":"Kosoy","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno B.","family":"Chomel","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henri-Jean","family":"Boulouis","sequence":"additional","affiliation":[{"name":"UMR BIPAR\/AFSSA, Institut National de la Recherche Agronomique, Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort\/UVPM, 7 avenue du G\u00e9n\u00e9ral de Gaulle, 94704 Maisons-Alfort Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichi","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1973","DOI":"10.1099\/00207713-50-6-1973","article-title":"Bartonella birtlesii sp. nov., isolated from small mammals ( Apodemus spp.)","volume":"50","author":"Bermond","year":"2000","journal-title":"Int J Syst Evol Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1099\/00207713-52-2-383","article-title":"Bartonella bovis Bermond et al. sp. nov. and Bartonella capreoli sp. nov., isolated from European ruminants","volume":"52","author":"Bermond","year":"2002","journal-title":"Int J Syst Evol Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1099\/00207713-46-4-891","article-title":"Comparison of partial citrate synthase gene ( gltA ) sequences for phylogenetic analysis of Bartonella species","volume":"46","author":"Birtles","year":"1996","journal-title":"Int J Syst Bacteriol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1099\/00207713-45-1-1","article-title":"Proposals to unify the genera Grahamella and Bartonella , with descriptions of Bartonella talpae comb. nov., Bartonella peromysci comb. nov., and three new species, Bartonella grahamii sp.nov., Bartonella taylorii sp. nov., and Bartonella doshiae sp. nov","volume":"45","author":"Birtles","year":"1995","journal-title":". 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