{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T17:18:44Z","timestamp":1774804724202,"version":"3.50.1"},"reference-count":12,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:p>Gram-negative, aerobic, motile, rod-shaped bacteria were isolated from the intestines of freshwater fish on two separate occasions. Colonies of both strains, JF3835<jats:sup>T<\/jats:sup> and JF4413, produced non-diffusible green pigment following 4\u20135\u2005days incubation on Luria\u2013Bertani agar. The most abundant fatty acids were summed feature 3 (comprising C<jats:sub>16\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic> and\/or C<jats:sub>15\u200a:\u200a0<\/jats:sub> iso 2-OH), C<jats:sub>16\u200a:\u200a0<\/jats:sub> and C<jats:sub>18\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u03c9<\/jats:italic>7<jats:italic>c.<\/jats:italic> The DNA G+C content was 62.9\u2005mol%. Sequence analysis of the 16S rRNA gene indicated 100\u200a% sequence similarity between the two strains. In comparison with recognized species, the new strains exhibited the greatest degree of sequence similarity with members of the <jats:italic>Pseudomonas chlororaphis<\/jats:italic> subspecies: <jats:italic>P. chlororaphis<\/jats:italic> subsp. <jats:italic>chlororaphis<\/jats:italic> (99.84\u200a%), <jats:italic>P. chlororaphis<\/jats:italic> subsp. <jats:italic>aurantiaca<\/jats:italic> (99.75\u200a%) and <jats:italic>P. chlororaphis<\/jats:italic> subsp. <jats:italic>aureofaciens<\/jats:italic> (99.40\u200a%). While DNA\u2013DNA relatedness confirmed the placement of strains JF3835<jats:sup>T<\/jats:sup> and JF4413 as members of the species <jats:italic>P. chlororaphis<\/jats:italic>, multilocus sequencing indicated that the strains formed a distinct cluster within it. On the basis of genotypic and phenotypic evidence, strains JF3835<jats:sup>T<\/jats:sup> and JF4413 represent a novel subspecies of the species <jats:italic>P. chlororaphis<\/jats:italic>, for which the name <jats:italic>Pseudomonas chlororaphis<\/jats:italic> subsp. <jats:italic>piscium<\/jats:italic> subsp. nov. is proposed. The type strain is JF3835<jats:sup>T<\/jats:sup> (=NCIMB 14478<jats:sup>T<\/jats:sup>=DSM 21509<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.011692-0","type":"journal-article","created":{"date-parts":[[2010,1,9]],"date-time":"2010-01-09T03:03:35Z","timestamp":1263006215000},"page":"2753-2757","source":"Crossref","is-referenced-by-count":39,"title":["Pseudomonas chlororaphis subsp. piscium subsp. nov., isolated from freshwater fish"],"prefix":"10.1099","volume":"60","author":[{"given":"Sarah E.","family":"Burr","sequence":"first","affiliation":[{"name":"Institute for Veterinary Bacteriology, Universit\u00e4t Bern, L\u00e4nggassstrasse 122, Postfach, CH-3001 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Gobeli","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Bacteriology, Universit\u00e4t Bern, L\u00e4nggassstrasse 122, Postfach, CH-3001 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Kuhnert","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Bacteriology, Universit\u00e4t Bern, L\u00e4nggassstrasse 122, Postfach, CH-3001 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elinor","family":"Goldschmidt-Clermont","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Bacteriology, Universit\u00e4t Bern, L\u00e4nggassstrasse 122, Postfach, CH-3001 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Frey","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Bacteriology, Universit\u00e4t Bern, L\u00e4nggassstrasse 122, Postfach, CH-3001 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1099\/00207713-39-3-224","article-title":"Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains","volume":"39","author":"Ezaki","year":"1989","journal-title":"Int J Syst Bacteriol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/j.1365-2761.2009.01021.x","article-title":"Pseudomonas chlororaphis strain JF3835 reduces mortality of juvenile perch ( Perca fluviatilis L.) caused by Aeromonas sobria","volume":"32","author":"Gobeli","year":"2009","journal-title":"J Fish Dis"},{"key":"R3","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1111\/j.1365-2761.2008.00912.x","article-title":"Identification of bacteria from the normal flora of perch, Perca fluviatilis L., and evaluation of their inhibitory potential towards Aeromonas species","volume":"31","author":"Goldschmidt-Clermont","year":"2008","journal-title":"J Fish Dis"},{"key":"R4","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1023\/B:ANTO.0000024910.57117.16","article-title":"Improved resolution on the phylogenetic relationships among Pseudomonas by the combined analysis of atp D, car A, rec A and 16S rDNA","volume":"86","author":"Hilario","year":"2004","journal-title":"Antonie van Leeuwenhoek"},{"key":"R5","first-page":"p","article-title":"Chapter 2: Introduction to Microbiology","volume-title":"In . 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