{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:13:10Z","timestamp":1792116790507,"version":"4.4.0"},"reference-count":25,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:p>\n                  <jats:italic>Acinetobacter<\/jats:italic> genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA\u2013DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names <jats:italic>Acinetobacter bereziniae<\/jats:italic> sp. nov. and <jats:italic>Acinetobacter guillouiae<\/jats:italic> sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP)  analysis <jats:italic>rpoB<\/jats:italic> sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption\/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA\u2013DNA reassociation. Results indicate that <jats:italic>A. bereziniae<\/jats:italic> and <jats:italic>A. guillouiae<\/jats:italic> represent two phenetically and phylogenetically distinct groups within the genus <jats:italic>Acinetobacter<\/jats:italic>. Based on the comparative analysis of housekeeping genes (16S rRNA and <jats:italic>rpoB<\/jats:italic> genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize <jats:sc>d<\/jats:sc>-glucose and to grow at 38\u2005\u00b0C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, <jats:italic>A. bereziniae<\/jats:italic> strains were positive for both tests, whereas <jats:italic>A. guillouiae<\/jats:italic> strains were negative in these tests. The strains of <jats:italic>A. bereziniae<\/jats:italic> originated mainly from human clinical specimens, whereas <jats:italic>A. guillouiae<\/jats:italic> strains were isolated from different environmental sources in addition to human specimens. The type strain of <jats:italic>A. bereziniae<\/jats:italic> sp. nov. is LMG 1003<jats:sup>T<\/jats:sup> (=CIP 70.12<jats:sup>T<\/jats:sup> =ATCC 17924<jats:sup>T<\/jats:sup>) and that of <jats:italic>A. guillouiae<\/jats:italic> sp. nov. is LMG 988<jats:sup>T<\/jats:sup> (=CIP 63.46<jats:sup>T<\/jats:sup> =ATCC 11171<jats:sup>T<\/jats:sup> =CCUG 2491<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.013656-0","type":"journal-article","created":{"date-parts":[[2009,11,3]],"date-time":"2009-11-03T22:56:16Z","timestamp":1257288976000},"page":"896-903","source":"Crossref","is-referenced-by-count":108,"title":["Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., to accommodate Acinetobacter genomic species 10 and 11, respectively"],"prefix":"10.1099","volume":"60","author":[{"given":"Alexandr","family":"Nemec","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Genetics, National Institute of Public Health, \u0160rob\u00e1rova 48, CZ-100 42 Prague 10, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Mus\u00edlek","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, \u0160rob\u00e1rova 48, CZ-100 42 Prague 10, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"\u0160edo","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5 (Building A2), CZ-625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"De Baere","sequence":"additional","affiliation":[{"name":"Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, 3 Blok A, De Pintelaan 185, B-9000 Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Maixnerov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Genetics, National Institute of Public Health, \u0160rob\u00e1rova 48, CZ-100 42 Prague 10, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanny J. K.","family":"van der Reijden","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center C5-P, PO Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zbyn\u011bk","family":"Zdr\u00e1hal","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5 (Building A2), CZ-625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Vaneechoutte","sequence":"additional","affiliation":[{"name":"Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, 3 Blok A, De Pintelaan 185, B-9000 Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenie","family":"Dijkshoorn","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center C5-P, PO Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1128\/JCM.38.11.4201-4207.2000","article-title":"Application of tRNA intergenic spacer PCR for identification of Enterococcus species","volume":"38","author":"Baele","year":"2000","journal-title":"J Clin Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1128\/JCM.39.4.1436-1442.2001","article-title":"Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species","volume":"39","author":"Baele","year":"2001","journal-title":"J Clin Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1128\/JB.95.5.1520-1541.1968","article-title":"A study of the Moraxella group. 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