{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T03:54:19Z","timestamp":1792900459459,"version":"4.5.0"},"reference-count":46,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"\n A Gram-positive bacterium, designated strain CBMB205\n T<\/jats:sup>\n , was isolated from the rhizosphere soil of traditionally cultivated, field-grown rice. Cells were strictly aerobic, motile, rod-shaped and formed endospores. The best growth was achieved at 30\u2005\u00b0C and pH\u20057.0 in ammonium mineral salts (AMS) medium containing 600\u2005mM methanol. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed strain CBMB205\n T<\/jats:sup>\n in a clade with the species\n Bacillus amyloliquefaciens<\/jats:italic>\n ,\n Bacillus vallismortis, Bacillus subtilis, Bacillus atrophaeus, Bacillus mojavensis<\/jats:italic>\n and\n Bacillus licheniformis<\/jats:italic>\n and revealed pairwise similarities ranging from 98.2 to 99.2\u200a%. DNA\u2013DNA hybridization experiments revealed a low level (<36\u200a%) of DNA\u2013DNA relatedness between strain CBMB205\n T<\/jats:sup>\n and its closest relatives. The major components of the fatty acid profile were C\n 15\u200a:\u200a0<\/jats:sub>\n anteiso, C\n 15\u200a:\u200a0<\/jats:sub>\n iso, C\n 16\u200a:\u200a0<\/jats:sub>\n iso and C\n 17\u200a:\u200a0<\/jats:sub>\n anteiso. The diagnostic diamino acid of the cell wall was\n meso<\/jats:italic>\n -diaminopimelic acid. The G+C content of the genomic DNA was 45.0\u2005mol%. The lipids present in strain CBMB205\n T<\/jats:sup>\n were diphosphatidylglycerol, phosphatidylglycerol, a minor amount of phosphatidylcholine and two unknown phospholipids. The predominant respiratory quinone was MK-7. Studies of DNA\u2013DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing enabled strain CBMB205\n T<\/jats:sup>\n to be described as representing a novel species of the genus\n Bacillus,<\/jats:italic>\n for which the name\n Bacillus methylotrophicus<\/jats:italic>\n sp. nov. is proposed. The type strain is CBMB205\n T<\/jats:sup>\n (=KACC 13105\n T<\/jats:sup>\n =NCCB 100236\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijs.0.015487-0","type":"journal-article","created":{"date-parts":[[2009,12,4]],"date-time":"2009-12-04T23:09:56Z","timestamp":1259968196000},"page":"2490-2495","source":"Crossref","is-referenced-by-count":137,"title":["Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil"],"prefix":"10.1099","volume":"60","author":[{"given":"Munusamy","family":"Madhaiyan","sequence":"first","affiliation":[{"name":"Biomaterials and Biocatalysts, Temasek Life Sciences Laboratory, The National University of Singapore, Singapore 117604"},{"name":"Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selvaraj","family":"Poonguzhali","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon-Wo","family":"Kwon","sequence":"additional","affiliation":[{"name":"Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong-Min","family":"Sa","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","volume-title":"The Biochemistry of Methylotrophs","author":"Anthony","year":"1982"},{"key":"R2","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1099\/00207713-42-3-439","article-title":"Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol utilizing, endospore-forming bacteria","volume":"42","author":"Arfman","year":"1992","journal-title":"Int J Syst Bacteriol"},{"key":"R3","volume-title":"Handbook of Microbiological Media","author":"Atlas","year":"1993"},{"key":"R4","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1139\/w01-062","article-title":"Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase","volume":"47","author":"Belimov","year":"2001","journal-title":"Can J Microbiol"},{"key":"R5","first-page":"p","article-title":"Bacterium methylicum (Loew) Migula. 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