{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:55:35Z","timestamp":1790913335375,"version":"4.1.0"},"reference-count":25,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:p>Four nocardioform bacterial strains isolated from clinical respiratory sources were characterized using a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence analyses, these strains were found to be 100\u200a% similar to each other and were shown to belong to the genus <jats:italic>Nocardia<\/jats:italic>. Chemotaxonomic data [major menaquinone: <jats:italic>\u03c9<\/jats:italic>-cyclic isoprene side chain MK-8(H<jats:sub>4<jats:italic>cycl<\/jats:italic>\n                  <\/jats:sub>); major polar lipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides; major fatty acids: monounsaturated fatty acids with a considerable amount of tuberculostearic acid; and mycolic acids (52\u201362 carbon atoms)] were consistent with the assignment of the novel strains to the genus <jats:italic>Nocardia<\/jats:italic>. Comparative phylogenetic analysis of the 16S rRNA gene sequences showed that the novel strains were related to <jats:italic>Nocardia cerradoensis<\/jats:italic> DSM 44546<jats:sup>T<\/jats:sup> (99.8\u200a%) and <jats:italic>Nocardia aobensis<\/jats:italic> JCM 12352<jats:sup>T<\/jats:sup> (99.6\u200a%). Analysis of <jats:italic>gyrB<\/jats:italic> gene sequences showed these strains were related to <jats:italic>N. aobensis<\/jats:italic> (96.6\u200a%) and to <jats:italic>N. cerradoensis<\/jats:italic> (96.3\u200a%). The results suggest that <jats:italic>gyrB<\/jats:italic> gene sequencing is a more powerful tool than 16S rRNA gene sequencing for taxonomic identification within the genus <jats:italic>Nocardia<\/jats:italic>. DNA\u2013DNA hybridization and physiological and biochemical tests supported the genotypic and phenotypic differentiation of the novel strains from related species. These data indicated that the new strains represent a novel species within the genus <jats:italic>Nocardia,<\/jats:italic> for which the name <jats:italic>Nocardia mikamii<\/jats:italic> sp. nov. is proposed, with strain W8061<jats:sup>T<\/jats:sup> (=DSM 45174<jats:sup>T<\/jats:sup>=JCM 15508<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijs.0.015594-0","type":"journal-article","created":{"date-parts":[[2009,11,14]],"date-time":"2009-11-14T02:58:03Z","timestamp":1258167483000},"page":"2272-2276","source":"Crossref","is-referenced-by-count":21,"title":["Nocardia mikamii sp. nov., isolated from human pulmonary infections in the USA"],"prefix":"10.1099","volume":"60","author":[{"given":"Deanna","family":"Jannat-Khah","sequence":"first","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial, and Mycotic Diseases National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 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