{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:23:34Z","timestamp":1790853814155,"version":"4.1.0"},"reference-count":39,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,9,1]]},"abstract":"<jats:p>Partial sequences of the<jats:italic>recN<\/jats:italic>gene (1249\u2005bp), which encodes a recombination and repair protein, were analysed to determine the phylogenetic relationship and identification of streptococci. The partial sequences presented interspecies nucleotide similarity of 56.4\u201398.2\u200a% and intersubspecies similarity of 89.8\u201398\u200a%. The mean DNA sequence similarity of<jats:italic>recN<\/jats:italic>gene sequences (66.6\u200a%) was found to be lower than those of the 16S rRNA gene (94.1\u200a%),<jats:italic>rpoB<\/jats:italic>(84.6\u200a%),<jats:italic>sodA<\/jats:italic>(74.8\u200a%),<jats:italic>groEL<\/jats:italic>(78.1\u200a%) and<jats:italic>gyrB<\/jats:italic>(73.2\u200a%). Phylogenetically derived trees revealed six statistically supported groups:<jats:italic>Streptococcus salivarius<\/jats:italic>,<jats:italic>S. equinus<\/jats:italic>,<jats:italic>S. hyovaginalis<\/jats:italic>\/<jats:italic>S. pluranimalium<\/jats:italic>\/<jats:italic>S. thoraltensis<\/jats:italic>,<jats:italic>S. pyogenes<\/jats:italic>,<jats:italic>S. mutans<\/jats:italic>and<jats:italic>S. suis<\/jats:italic>. The \u2018mitis\u2019 group was not supported by a significant bootstrap value, but three statistically supported subgroups were noted:<jats:italic>Streptococcus sanguinis<\/jats:italic>\/<jats:italic>S. cristatus<\/jats:italic>\/<jats:italic>S. sinensis<\/jats:italic>,<jats:italic>S. anginosus\/S. intermedius\/S. constellatus<\/jats:italic>(the \u2018anginosus\u2019 subgroup) and<jats:italic>S. mitis<\/jats:italic>\/<jats:italic>S. infantis<\/jats:italic>\/<jats:italic>S. peroris<\/jats:italic>\/<jats:italic>S. oralis<\/jats:italic>\/<jats:italic>S. oligofermentans<\/jats:italic>\/<jats:italic>S. pneumoniae<\/jats:italic>\/<jats:italic>S. pseudopneumoniae<\/jats:italic>. The partial<jats:italic>recN<\/jats:italic>gene sequence comparison highlighted a high percentage of divergence between<jats:italic>Streptococcus dysgalactiae<\/jats:italic>subsp.<jats:italic>dysgalactiae<\/jats:italic>and<jats:italic>S. dysgalactiae<\/jats:italic>subsp.<jats:italic>equisimilis<\/jats:italic>. This observation is confirmed by other gene sequence comparisons (<jats:italic>groEL<\/jats:italic>,<jats:italic>gyrB<\/jats:italic>,<jats:italic>rpoB<\/jats:italic>and<jats:italic>sodA<\/jats:italic>). A high percentage of similarity was found between<jats:italic>S. intermedius<\/jats:italic>and<jats:italic>S. constellatus<\/jats:italic>after sequence comparison of the<jats:italic>recN<\/jats:italic>gene. To study the genetic diversity among the \u2018anginosus\u2019 subgroup,<jats:italic>recN<\/jats:italic>,<jats:italic>groEL<\/jats:italic>,<jats:italic>sodA<\/jats:italic>,<jats:italic>gyrB<\/jats:italic>and<jats:italic>rpoB<\/jats:italic>sequences were determined for 36 clinical isolates. The results that were obtained confirmed the high genetic diversity within this group of streptococci.<\/jats:p>","DOI":"10.1099\/ijs.0.018176-0","type":"journal-article","created":{"date-parts":[[2009,10,31]],"date-time":"2009-10-31T01:39:46Z","timestamp":1256953186000},"page":"2140-2148","source":"Crossref","is-referenced-by-count":62,"title":["Partial recN gene sequencing: a new tool for identification and phylogeny within the genus Streptococcus"],"prefix":"10.1099","volume":"60","author":[{"given":"Olga O.","family":"Glazunova","sequence":"first","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie \u2013 Virologie, H\u00f4pital de la Timone, CNRS UMR 6236, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie \u2013 Virologie, H\u00f4pital de la Timone, CNRS UMR 6236, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Roux","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie \u2013 Virologie, H\u00f4pital de la Timone, CNRS UMR 6236, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1128\/JCM.42.6.2651-2657.2004","article-title":"Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S\u201323S ribosomal DNA spacer region","volume":"42","author":"Chen","year":"2004","journal-title":"J Clin Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1099\/00207713-37-3-222","article-title":"\u201c Streptococcus milleri \u201d, Streptococcus constellatus , and Streptococcus intermedius are later synonyms of Streptococcus anginosus","volume":"37","author":"Coykendall","year":"1987","journal-title":"Int J Syst Bacteriol"},{"key":"R4","article-title":"Streptococcus Rosenbach 1884, genus","volume-title":"In List of Bacterial Names with Standing in Nomenclature","author":"Euz\u00e9by","year":"2010"},{"key":"R5","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1128\/CMR.15.4.613-630.2002","article-title":"What happened to the streptococci: overview of taxonomic and nomenclature changes","volume":"15","author":"Facklam","year":"2002","journal-title":"Clin Microbiol Rev"},{"key":"R6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.2307\/2412028","article-title":"Methods for computing Wagner trees","volume":"19","author":"Farris","year":"1970","journal-title":"Syst Zool"},{"key":"R7","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1096-0031.1994.tb00181.x","article-title":"Testing significance of incongruence","volume":"10","author":"Farris","year":"1994","journal-title":"Cladistics"},{"key":"R8","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/S0723-2020(84)80005-3","article-title":"Taxonomic studies on streptococci of serological groups C, G and L and possibly related taxa","volume":"5","author":"Farrow","year":"1984","journal-title":"Syst Appl Microbiol"},{"key":"R9","first-page":"164","article-title":"phylip \u2013 phylogeny inference package (version 3.2)","volume":"5","author":"Felsenstein","year":"1989","journal-title":"Cladistics"},{"key":"R10","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.1128\/JCM.35.9.2337-2341.1997","article-title":"Identification of clinically relevant viridans group streptococci to the species level by PCR","volume":"35","author":"Garnier","year":"1997","journal-title":"J Clin Microbiol"},{"key":"R11","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1099\/00207713-33-2-404","article-title":"Streptococcus dysgalactiae (Diernhofer) nom","volume":"33","author":"Garvie","year":"1983","journal-title":"rev. 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