{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:43:17Z","timestamp":1790818997435,"version":"4.1.0"},"reference-count":31,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:p>A yellow-pigmented bacterial strain, R4-1A<jats:sup>T<\/jats:sup>, isolated from the midgut of the mosquito <jats:italic>Culex quinquefasciatus<\/jats:italic> (a vector of lymphatic filariasis), was studied using a polyphasic approach. Cells of the isolate were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence of this organism with sequences of type strains of the most closely related species clearly showed an allocation to the genus <jats:italic>Chryseobacterium<\/jats:italic>, with the highest sequence similarities (all 97.9\u200a%) to <jats:italic>Chryseobacterium jejuense<\/jats:italic> JS17-8<jats:sup>T<\/jats:sup>, <jats:italic>C. indologenes<\/jats:italic> ATCC 29897<jats:sup>T<\/jats:sup>, <jats:italic>C. arthrosphaerae<\/jats:italic> CC-VM-7<jats:sup>T<\/jats:sup> and <jats:italic>C. aquifrigidense<\/jats:italic> CW9<jats:sup>T<\/jats:sup>. 16S rRNA gene sequence similarities to type strains of other <jats:italic>Chryseobacterium<\/jats:italic> species were below 97.5\u200a%. The fatty acid profile of strain R4-1A<jats:sup>T<\/jats:sup> included the major fatty acids iso-15\u200a:\u200a0, summed feature 4 (comprising iso-15\u200a:\u200a0 2-OH and\/or 16\u200a:\u200a1<jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>), iso-17\u200a:\u200a1<jats:italic>\u03c99c<\/jats:italic> and iso-17\u200a:\u200a0 3-OH. DNA\u2013DNA hybridizations with <jats:italic>C. jejuense<\/jats:italic> KACC 12501<jats:sup>T<\/jats:sup>, <jats:italic>C. indologenes<\/jats:italic> CCUG 14556<jats:sup>T<\/jats:sup>, <jats:italic>C. arthrosphaerae<\/jats:italic> CC-VM-7<jats:sup>T<\/jats:sup> and <jats:italic>C. aquifrigidense<\/jats:italic> KCTC 12894<jats:sup>T<\/jats:sup> resulted in relatedness values of 38.3\u200a% (reciprocal 30.5\u200a%), 29.4\u200a% (32.1\u200a%), 23.2\u200a% (37.2\u200a%) and 29.5\u200a% (47.1\u200a%), respectively. These results and the differentiating biochemical and chemotaxonomic properties show that strain R4-1A<jats:sup>T<\/jats:sup> represents a novel species, for which the name <jats:italic>Chryseobacterium culicis<\/jats:italic> sp. nov. is proposed. The type strain is R4-1A<jats:sup>T<\/jats:sup> (=LMG 25442<jats:sup>T<\/jats:sup> =CCM 7716<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.019794-0","type":"journal-article","created":{"date-parts":[[2009,11,21]],"date-time":"2009-11-21T02:37:29Z","timestamp":1258771049000},"page":"2387-2391","source":"Crossref","is-referenced-by-count":37,"title":["Chryseobacterium culicis sp. nov., isolated from the midgut of the mosquito Culex quinquefasciatus"],"prefix":"10.1099","volume":"60","author":[{"given":"Peter","family":"K\u00e4mpfer","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Angewandte Mikrobiologie, Justus-Liebig-Universit\u00e4t Giessen, Heinrich-Buff-Ring 36\u201332, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kshitij","family":"Chandel","sequence":"additional","affiliation":[{"name":"Defense Research and Development Establishment, Jhansi Road, Gwalior 474 002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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S.","family":"Shouche","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science, Pune 411 007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay","family":"Veer","sequence":"additional","affiliation":[{"name":"Defense Research and Development Establishment, Jhansi Road, Gwalior 474 002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1099\/ijs.0.65544-0","article-title":"Chryseobacterium gregarium sp. nov., isolated from decaying plant material","volume":"58","author":"Behrendt","year":"2008","journal-title":"Int J Syst Evol Microbiol"},{"issue":"7","key":"R2","first-page":"638","article-title":"The genera Chryseobacterium and Elizabethkingia","volume-title":"In The Prokaryotes, a Handbook on the Biology of 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