{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T01:16:41Z","timestamp":1792891001373,"version":"4.5.0"},"reference-count":26,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,2,1]]},"abstract":"<jats:p>Members of the genus <jats:italic>Nocardia<\/jats:italic> are responsible for cutaneous, pulmonary and disseminated human infections. From 2003 to 2008, four nocardioform strains (W8027, W8681, W9071 and W9241<jats:sup>T<\/jats:sup>) were isolated from patients in the state of Florida, USA. Ribosomal gene sequencing analysis suggested that a novel species of the genus <jats:italic>Nocardia<\/jats:italic> had been isolated. These strains were subjected to a taxonomic analysis using a polyphasic approach. Phenotypic analyses included morphological examination, biochemical profiling and antimicrobial susceptibility testing. Molecular studies included 16S rRNA and DNA gyrase B subunit (<jats:italic>gyrB)<\/jats:italic> gene sequence analyses and DNA\u2013DNA hybridization. Phylogenetic neighbours were determined through 16S rRNA and <jats:italic>gyrB<\/jats:italic> gene sequence analyses. Phenotypic characteristics that differentiated the novel isolates from phylogenetically related species were growth at 45\u2005\u00b0C, and three of the four novel strains utilized <jats:sc>l<\/jats:sc>-rhamnose. The antimicrobial profiles could not reliably distinguish the novel species from related nocardiae. Analysis showed that the 16S rRNA gene sequences of the four novel isolates were identical. The <jats:sc>blast<\/jats:sc> analysis of the near full-length 16S rRNA gene showed 99.2\u200a% sequence similarity to <jats:italic>Nocardia araoensis<\/jats:italic> DSM 44729<jats:sup>T<\/jats:sup>, <jats:italic>Nocardia arthritidis<\/jats:italic> DSM 44731<jats:sup>T<\/jats:sup> and <jats:italic>Nocardia beijingensis<\/jats:italic> JCM 10666<jats:sup>T<\/jats:sup>, 98.7\u200a% to <jats:italic>Nocardia amamiensis<\/jats:italic> DSM 45066<jats:sup>T<\/jats:sup>, 98.2\u200a% to <jats:italic>Nocardia pneumoniae<\/jats:italic> JCM 12119<jats:sup>T<\/jats:sup> and 97.8\u200a% to <jats:italic>Nocardia takedensis<\/jats:italic> JCM 13313<jats:sup>T<\/jats:sup>. Analysis of partial <jats:italic>gyrB<\/jats:italic> gene sequences showed that the novel isolates had 95.4\u200a% similarity to <jats:italic>N. arthritidis<\/jats:italic> DSM 44731<jats:sup>T<\/jats:sup>, 95.3\u200a% to <jats:italic>Nocardia gamkensis<\/jats:italic> DSM 44956<jats:sup>T<\/jats:sup>, 94.4\u200a% to <jats:italic>N. pneumoniae<\/jats:italic> JCM 12119<jats:sup>T<\/jats:sup>, 93.8\u200a% to <jats:italic>Nocardia asiatica<\/jats:italic> DSM 44668<jats:sup>T<\/jats:sup>, 93.5\u200a% to <jats:italic>N. amamiensis<\/jats:italic> DSM 45066<jats:sup>T<\/jats:sup>, 93.4\u200a% to <jats:italic>N. beijingensis<\/jats:italic> JCM 10666<jats:sup>T<\/jats:sup> and 93.2\u200a% to <jats:italic>N. araoensis<\/jats:italic> DSM 44729<jats:sup>T<\/jats:sup>. The DNA\u2013DNA relatedness values between the four novel strains were 86\u201389\u200a%; the relatedness value for strain W9241<jats:sup>T<\/jats:sup> compared with <jats:italic>N. beijingensis<\/jats:italic> JCM 10666<jats:sup>T<\/jats:sup> was 47\u200a% and 46\u200a% with <jats:italic>N. araoensis<\/jats:italic> DSM 44729<jats:sup>T<\/jats:sup>, 44\u200a% with <jats:italic>N. arthritidis<\/jats:italic> DSM 44731<jats:sup>T<\/jats:sup>, 32\u200a% with <jats:italic>N. amamiensis<\/jats:italic> DSM 45066<jats:sup>T<\/jats:sup> and 20\u200a% with <jats:italic>N. asiatica<\/jats:italic> DSM 44668<jats:sup>T<\/jats:sup>. The results of the taxonomic analysis suggested that the new isolates represent a novel species of the genus <jats:italic>Nocardia<\/jats:italic> for which the name <jats:italic>Nocardia niwae<\/jats:italic> sp. nov. is proposed. The type strain is W9241<jats:sup>T<\/jats:sup> (=DSM 45340<jats:sup>T<\/jats:sup>=CCUG 57756<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.020370-0","type":"journal-article","created":{"date-parts":[[2010,3,27]],"date-time":"2010-03-27T03:09:09Z","timestamp":1269659349000},"page":"438-442","source":"Crossref","is-referenced-by-count":23,"title":["Nocardia niwae sp. nov., isolated from human pulmonary sources"],"prefix":"10.1099","volume":"61","author":[{"given":"Benjamin D.","family":"Moser","sequence":"first","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Klenk","sequence":"additional","affiliation":[{"name":"German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Schumann","sequence":"additional","affiliation":[{"name":"German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"P\u00f6tter","sequence":"additional","affiliation":[{"name":"German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent A.","family":"Lasker","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold G.","family":"Steigerwalt","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, 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