{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:34:37Z","timestamp":1785497677490,"version":"3.56.0"},"reference-count":21,"publisher":"Microbiology Society","issue":"7","funder":[{"name":"National High Technology Research and Development Program of China","award":["2006AA10Z402"],"award-info":[{"award-number":["2006AA10Z402"]}]},{"name":"Natural Science Foundation of Jiangsu Province, China","award":["BK2008331"],"award-info":[{"award-number":["BK2008331"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30970099"],"award-info":[{"award-number":["30970099"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:p>The taxonomic status was determined of an aerobic, facultatively methylotrophic strain, JZL-4<jats:sup>T<\/jats:sup>, isolated from activated sludge. The cells were Gram-negative, asporogenous, colourless, motile, short rods. The strain utilized methanol, methylamine, formate and a variety of polycarbon compounds, but not methane, dichloromethane or CO<jats:sub>2<\/jats:sub>\/H<jats:sub>2<\/jats:sub>, as carbon and energy sources. C<jats:sub>1<\/jats:sub> compounds were assimilated via the isocitrate lyase-negative serine pathway. Optimal growth occurred at 30 \u00b0C, pH 6.5\u20137.5 and 0.5\u200a% (w\/v) NaCl. The major cellular fatty acids were C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic> and C<jats:sub>18\u200a:\u200a0<\/jats:sub>. The major phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine (PME); PME, the main phospholipid of strain JZL-4<jats:sup>T<\/jats:sup>, was absent or present in only minor amounts in <jats:italic>Methylopila capsulata<\/jats:italic> IM1<jats:sup>T<\/jats:sup>, <jats:italic>Methylopila helvetica<\/jats:italic> DM9<jats:sup>T<\/jats:sup> and <jats:italic>Albibacter methylovorans<\/jats:italic> DM10<jats:sup>T<\/jats:sup>. The major ubiquinone was Q-10. The DNA G+C content of strain JZL-4<jats:sup>T<\/jats:sup> was 70.4 mol% (<jats:italic>T<\/jats:italic>\n                  <jats:sub>m<\/jats:sub>). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain showed high sequence similarities to <jats:italic>M. capsulata<\/jats:italic> IM1<jats:sup>T<\/jats:sup> (97.2\u200a%), <jats:italic>A. methylovorans<\/jats:italic> DM10<jats:sup>T<\/jats:sup> (94.9\u200a%) and <jats:italic>M. helvetica<\/jats:italic> DM9<jats:sup>T<\/jats:sup> (94.1\u200a%), and showed less than 94\u200a% similarity to strains of other species with validly published names. Strain JZL-4<jats:sup>T<\/jats:sup> had a low level of DNA\u2013DNA relatedness (34\u200a%) with <jats:italic>M. capsulata<\/jats:italic> IM1<jats:sup>T<\/jats:sup>. On the basis of phenotypic, genetic and phylogenetic data, strain JZL-4<jats:sup>T<\/jats:sup> is proposed to represent a novel species of the genus <jats:italic>Methylopila<\/jats:italic>, with the name <jats:italic>Methylopila jiangsuensis<\/jats:italic> sp. nov. The type strain is strain JZL-4<jats:sup>T<\/jats:sup> (\u200a=\u200aACCC 05406<jats:sup>T<\/jats:sup> \u200a=\u200aDSM 22718<jats:sup>T<\/jats:sup> \u200a=\u200aVKM B-2555<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.020925-0","type":"journal-article","created":{"date-parts":[[2010,7,24]],"date-time":"2010-07-24T01:48:57Z","timestamp":1279936137000},"page":"1561-1566","source":"Crossref","is-referenced-by-count":13,"title":["Methylopila jiangsuensis sp. nov., an aerobic, facultatively methylotrophic bacterium"],"prefix":"10.1099","volume":"61","author":[{"given":"Lian","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Wei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao-Jian","family":"Hang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina V.","family":"Doronina","sequence":"additional","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri A.","family":"Trotsenko","sequence":"additional","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun-Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1128\/AEM.44.4.992-993.1982","article-title":"Nonstaining (KOH) method for determination of gram reactions of marine bacteria","volume":"44","author":"Buck","year":"1982","journal-title":"Appl Environ Microbiol"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64915-0"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1970.tb00830.x"},{"key":"r4","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S0723-2020(11)80454-6","article-title":"Isolation and characterization of a new facultatively methylotrophic bacterium: description of Methylorhabdus multivorans gen. nov., sp. nov.","volume":"18","author":"Doronina","year":"1995","journal-title":"Syst Appl Microbiol"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-4-1313"},{"key":"r6","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0723-2020(00)80007-7","article-title":"Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov. \u2013 novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane","volume":"23","author":"Doronina","year":"2000","journal-title":"Syst Appl Microbiol"},{"key":"r7","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1099\/00207713-51-3-1051","article-title":"Albibacter methylovorans gen. nov., sp. nov., a novel aerobic, facultatively autotrophic and methylotrophic bacterium that utilizes dichloromethane","volume":"51","author":"Doronina","year":"2001","journal-title":"Int J Syst Evol Microbiol"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1104\/pp.26.1.192"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"r11","first-page":"115","article-title":"16S\/23S rRNA sequencing","volume-title":"Nucleic Acid Techniques in Bacterial Systematics","author":"Lane","year":"1991"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(62)80066-7"},{"key":"r13","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol Biol Evol"},{"key":"r14","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"r15","author":"Sasser","year":"1990","journal-title":"Identification of bacteria by gas chromatography of cellular fatty acids. 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