{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:49:51Z","timestamp":1790880591562,"version":"4.1.0"},"reference-count":48,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,1,1]]},"abstract":"<jats:p>A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8<jats:sup>T<\/jats:sup>, was isolated from an acidic (pH\u20054.0\u20134.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2\u20130.3\u2005\u03bcm in diameter and 0.8\u20133.0\u2005\u03bcm long) and irregular cocci (0.2\u20130.8\u2005\u03bcm in diameter). The culture utilized H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub> to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH\u20055.1 and 35\u2005\u00b0C. The culture grew in basal medium containing as little as 0.43\u2005mM Na<jats:sup>+<\/jats:sup> and growth was inhibited completely by 50\u2005mM NaCl. To our knowledge, strain 6A8<jats:sup>T<\/jats:sup> is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, <jats:italic>Methanoregula boonei<\/jats:italic> gen. nov., sp. nov. The type strain of <jats:italic>Methanoregula boonei<\/jats:italic> is 6A8<jats:sup>T<\/jats:sup> (=DSM 21154<jats:sup>T<\/jats:sup> =JCM 14090<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.021782-0","type":"journal-article","created":{"date-parts":[[2010,2,13]],"date-time":"2010-02-13T03:10:45Z","timestamp":1266030645000},"page":"45-52","source":"Crossref","is-referenced-by-count":192,"title":["Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog"],"prefix":"10.1099","volume":"61","author":[{"given":"Suzanna L.","family":"Br\u00e4uer","sequence":"first","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hinsby","family":"Cadillo-Quiroz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah J.","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph B.","family":"Yavitt","sequence":"additional","affiliation":[{"name":"Department of Natural Resources, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Zinder","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/MMBR.43.2.260-296.1979","article-title":"Methanogens: reevaluation of a unique biological group","volume":"43","author":"Balch","year":"1979","journal-title":"Microbiol Rev"},{"key":"R2","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1080\/713851165","article-title":"Methane biogeochemistry and methanogen communities in two northern peatland ecosystems, New York State","volume":"20","author":"Basiliko","year":"2003","journal-title":"Geomicrobiol J"},{"key":"R3","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1139\/m86-025","article-title":"Methanococcus frisius sp. nov., a new methylotrophic marine methanogen","volume":"32","author":"Blotevogel","year":"1986","journal-title":"Can J Microbiol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1099\/00207713-38-2-212","article-title":"Proposal of minimal standards for describing new taxa of methanogenic bacteria","volume":"38","author":"Boone","year":"1988","journal-title":"Int J Syst Bacteriol"},{"issue":"1","key":"R5","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/978-0-387-21609-6","article-title":"Order II. 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