{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:16:56Z","timestamp":1792117016096,"version":"4.4.0"},"reference-count":27,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,11,1]]},"abstract":"<jats:p>Cells of strain LX3<jats:sup>T<\/jats:sup>, isolated from soil, were Gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10\u200a\u00b0C, unable to produce gas from lactose at 45\u200a\u00b0C and unable to produce indole. The isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. The G+C content of the DNA was 56\u00b74\u2005mol%. Furthermore, comparison of 16S rRNA and <jats:italic>rpoB<\/jats:italic> gene sequences showed that the isolate clearly belongs to the genus <jats:italic>Klebsiella<\/jats:italic>. The closest phylogenetic relative was <jats:italic>Klebsiella pneumoniae<\/jats:italic>, there being 99\u00b73 and 97\u00b75\u200a% similarity in 16S rRNA and <jats:italic>rpoB<\/jats:italic> gene sequences, respectively. DNA\u2013DNA hybridization analysis demonstrated a very low level of relatedness to other members of the genus <jats:italic>Klebsiella<\/jats:italic>, indicating that the isolated strain and other species in the genus <jats:italic>Klebsiella<\/jats:italic> were not related at the species level. The isolate could be differentiated from other previously described members of the genus <jats:italic>Klebsiella<\/jats:italic> on the basis of phenotypic differences and 16S rRNA and <jats:italic>rpoB<\/jats:italic> gene sequence divergence, together with DNA\u2013DNA reassociation data. Therefore, it is proposed that strain LX3<jats:sup>T<\/jats:sup> (=DSM 16265<jats:sup>T<\/jats:sup>=JCM 12419<jats:sup>T<\/jats:sup>) should be classified as the type strain of a novel species of genus <jats:italic>Klebsiella<\/jats:italic>, <jats:italic>Klebsiella singaporensis<\/jats:italic> sp. nov.<\/jats:p>","DOI":"10.1099\/ijs.0.02690-0","type":"journal-article","created":{"date-parts":[[2004,11,15]],"date-time":"2004-11-15T23:14:12Z","timestamp":1100560452000},"page":"2131-2136","source":"Crossref","is-referenced-by-count":66,"title":["Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium"],"prefix":"10.1099","volume":"54","author":[{"given":"Xianzhen","family":"Li","sequence":"first","affiliation":[{"name":"Department of Bio and Food Engineering, Dalian College of Light Industry, Dalian 116034, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daohai","family":"Zhang","sequence":"additional","affiliation":[{"name":"IMA, National 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