{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:42:15Z","timestamp":1791182535414,"version":"4.1.0"},"reference-count":18,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:p>A novel <jats:italic>Legionella<\/jats:italic> species was identified based on analysis of 16S rRNA and <jats:italic>mip<\/jats:italic> (macrophage infectivity potentiator) gene sequences, cellular fatty acids, isoprenoid quinones, biochemical reactions, antigens and quantitative DNA\u2013DNA hybridization. Strain CDC-1796-JAP-E<jats:sup>T<\/jats:sup> was isolated from well water at the Nagasaki Municipal Medical Center, Japan. Two strains, CDC-3041-AUS-E and CDC-3558-AUS-E, were isolated from water samples during an outbreak of legionellosis in South Australia. Strain CDC-5427-OH-H was isolated from a 66-year-old female patient diagnosed with Legionnaires\u2019 disease in the US. Cells from these four strains were Gram-negative, non-fluorescent, rod-shaped, and positive for alkaline phosphatase, esterase, leucine arylamidase, catalase, gelatinase, \u03b2-lactamase and tyrosine browning assay. Phylogenetic analysis of 16S rRNA and <jats:italic>mip<\/jats:italic> genes revealed that the four strains formed a distinct cluster within the genus <jats:italic>Legionella.<\/jats:italic> The bacteria contained branched-chain fatty acids and quinones that are typical of members of the genus <jats:italic>Legionella<\/jats:italic>. Slide agglutination tests demonstrated no cross-reaction with 52 previously described members of the <jats:italic>Legionellaceae<\/jats:italic>. DNA\u2013DNA hybridization studies indicated that DNAs from the four strains were highly related (78\u201384\u200a%) but they showed 29\u200a% relatedness to <jats:italic>Legionella oakridgensis<\/jats:italic> ATCC 33761<jats:sup>T<\/jats:sup> and less than 10\u200a% to strains of other <jats:italic>Legionella<\/jats:italic> species tested. These characterizations suggest that the isolates represent a novel species, for which the name <jats:italic>Legionella nagasakiensis<\/jats:italic> sp. nov. is proposed; the type strain is CDC-1796-JAP-E<jats:sup>T<\/jats:sup> (\u200a=\u200aATCC BAA-1557<jats:sup>T<\/jats:sup>\u200a=\u200aJCM 15315<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.027193-0","type":"journal-article","created":{"date-parts":[[2011,3,14]],"date-time":"2011-03-14T00:07:43Z","timestamp":1300061263000},"page":"284-288","source":"Crossref","is-referenced-by-count":23,"title":["Legionella nagasakiensis sp. nov., isolated from water samples and from a patient with pneumonia"],"prefix":"10.1099","volume":"62","author":[{"given":"Genyan","family":"Yang","sequence":"first","affiliation":[{"name":"Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA"},{"name":"Division of Blood Disorders, National Center on Birth Defects and Developmental Disabilities, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Benson","sequence":"additional","affiliation":[{"name":"Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodney M.","family":"Ratcliff","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases, Institute of Medical and Veterinary Science, SA Pathology, and School of Molecular & Biomedical Science, University of Adelaide, Adelaide, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen W.","family":"Brown","sequence":"additional","affiliation":[{"name":"Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold G.","family":"Steigerwalt","sequence":"additional","affiliation":[{"name":"Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Lanier","family":"Thacker","sequence":"additional","affiliation":[{"name":"Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam I.","family":"Daneshvar","sequence":"additional","affiliation":[{"name":"Laboratory Science, Policy, and Practice Program Office; Office of Surveillance, Epidemiology and Laboratory Services, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger E.","family":"Morey","sequence":"additional","affiliation":[{"name":"Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Nagasaki University Hospital, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry S.","family":"Fields","sequence":"additional","affiliation":[{"name":"Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-3-631"},{"key":"r2","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1128\/JCM.13.4.714-719.1981","article-title":"Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium","volume":"13","author":"Bopp","year":"1981","journal-title":"J Clin 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