{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T01:35:46Z","timestamp":1787794546807,"version":"build-2784847793"},"reference-count":54,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,1,1]]},"abstract":"<jats:p>Strain K11<jats:sup>T<\/jats:sup>was isolated from activated sludge of a municipal wastewater-treatment plant. Phylogenetic analysis of the 16S rRNA gene sequence revealed that it represents a distinct line of descent within the<jats:italic>Comamonadaceae<\/jats:italic>. The novel strain was a Gram-negative, catalase- and oxidase-positive, non-motile, straight to slightly curved rod. Polyhydroxyalkanoate granules were stored intracellularly as reserve material. Colonies on agar plates were small, regular and characterized by a water-insoluble yellow pigment. Unbranched fatty acids 16\u200a:\u200a1<jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>, 16\u200a:\u200a0 and 18\u200a:\u200a1<jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>dominated the cellular fatty acid pattern and ubiquinone-8 (Q-8) was the major component of the respiratory lipoquinones, both traits typical of members of the<jats:italic>Comamonadaceae<\/jats:italic>. A distinguishing characteristic was the presence of the two hydroxy fatty acids 10\u200a:\u200a0 3-OH and 12\u200a:\u200a0 2-OH, each in significant amounts. The G+C content of the DNA was 59\u2005mol%. Strain K11<jats:sup>T<\/jats:sup>was capable of aerobic chemolithoheterotrophic growth using thiosulfate as an additional substrate, but could not grow autotrophically with thiosulfate or hydrogen. Facultative anaerobic growth was possible with nitrate and nitrite as electron acceptors, but not with ferric iron, sulfate or by fermentation. The sole end product of denitrification was N<jats:sub>2<\/jats:sub>O; nitrite accumulated only transiently in small amounts. Based upon phylogenetic and phenotypic evidence, it is proposed to establish the novel taxon<jats:italic>Ottowia thiooxydans<\/jats:italic>gen. nov., sp. nov., represented by the type strain K11<jats:sup>T<\/jats:sup>(=DSM 14619<jats:sup>T<\/jats:sup>=JCM 11629<jats:sup>T<\/jats:sup>).<jats:italic>Aquaspirillum gracile<\/jats:italic>was among the phylogenetically most closely related species to strain K11<jats:sup>T<\/jats:sup>. This species has been wrongly classified, and it is proposed to reclassify it as<jats:italic>Hylemonella gracilis<\/jats:italic>gen. nov., comb. nov. 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