{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T12:07:30Z","timestamp":1765368450486},"reference-count":52,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,3,1]]},"abstract":"Characterization of strains of Xanthomonas axonopodis<\/jats:italic> pv. citri by using DNA fingerprints that were generated from primers for enterobacterial repetitive intergenic consensus (ERIC) elements led to the discovery of differential sequences for a leucine-responsive regulatory protein (lrp<\/jats:italic>) gene in two subgroups of strains with different host ranges on Citrus<\/jats:italic> spp. DNA hybridization and PCR-based assays that used different sets of primers were designed to detect the core sequence, as well as to obtain the entire sequence of the lrp<\/jats:italic> gene for several Xanthomonas<\/jats:italic> species and pathovars. Higher variability was observed at the nucleotide level than at the amino acid level among the different species and pathovars, revealing selection pressure on the lrp<\/jats:italic> gene, which is presumably due to an essential role of the gene in bacterial metabolism. Moderate variability in the 3\u2032 and 5\u2032 domains was used to study relationships among different species within the genus Xanthomonas<\/jats:italic>. Species of this genus that were isolated from citrus, as well as other pathovars of X. axonopodis<\/jats:italic>, showed highly similar lrp<\/jats:italic> gene sequences, whereas other Xanthomonas<\/jats:italic> species, especially Xanthomonas campestris<\/jats:italic>, had sequences that were more dissimilar to that of X. axonopodis<\/jats:italic>. Thus, the lrp<\/jats:italic> gene sequence is useful to distinguish X. axonopodis<\/jats:italic> pv. citri groups and promising for polyphasic taxonomic analysis of the genus Xanthomonas<\/jats:italic>. Data from analysis of lrp<\/jats:italic> gene sequences support the current concepts for classification of xanthomonads, which are based on other approaches.<\/jats:p>","DOI":"10.1099\/ijs.0.02784-0","type":"journal-article","created":{"date-parts":[[2004,3,15]],"date-time":"2004-03-15T22:48:02Z","timestamp":1079390882000},"page":"429-437","source":"Crossref","is-referenced-by-count":30,"title":["The leucine-responsive regulatory protein (lrp) gene for characterization of the relationship among Xanthomonas species"],"prefix":"10.1099","volume":"54","author":[{"given":"Jaime","family":"Cubero","sequence":"first","affiliation":[{"name":"University of Florida, Citrus Research and Education Center (CREC), 700 Experiment Station Road, Lake Alfred, FL 33850-2299, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James H.","family":"Graham","sequence":"additional","affiliation":[{"name":"University of Florida, Citrus Research and Education Center (CREC), 700 Experiment Station Road, Lake Alfred, FL 33850-2299, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1094\/PHYTO.1999.89.4.328","article-title":"Multiphasic analysis of xanthomonads causing bacterial spot disease on tomato and pepper in the Caribbean and Central America: evidence for common lineages within and between countries","volume":"89","author":"Bouzar","year":"1999","journal-title":"Phytopathology"},{"key":"R2","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1128\/MMBR.58.3.466-490.1994","article-title":"The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli","volume":"58","author":"Calvo","year":"1994","journal-title":"Microbiol Rev"},{"key":"R3","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/S0022-2836(02)00187-0","article-title":"Leucine-induced dissociation of Escherichia coli Lrp hexadecamers to octamers","volume":"318","author":"Chen","year":"2002","journal-title":"J Mol Biol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1128\/AEM.68.3.1257-1264.2002","article-title":"Genetic relationships among worldwide strains of Xanthomonas causing canker in citrus species and design of new primers for their identification by PCR","volume":"68","author":"Cubero","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/417459a","article-title":"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities","volume":"417","author":"da Silva","year":"2002","journal-title":"Nature"},{"key":"R6","first-page":"243","article-title":"Genus II. 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