{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:49:52Z","timestamp":1788871792957,"version":"build-2803163510"},"reference-count":31,"publisher":"Microbiology Society","issue":"10","funder":[{"name":"Ministry of Economy, Trade and Industry (METI) of Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,10,1]]},"abstract":"<jats:p>\n                    A methanogenic organism, designated strain HB-1\n                    <jats:sup>T<\/jats:sup>\n                    , from the domain Archaea was isolated from groundwater sampled from a subsurface Miocene formation located in Horonobe, Hokkaido, Japan. The strain grew on methanol, dimethylamine, trimethylamine, dimethylsulfide and acetate but not on monomethylamine, H\n                    <jats:sub>2<\/jats:sub>\n                    \/CO\n                    <jats:sub>2<\/jats:sub>\n                    , formate, 2-propanol, 2-butanol or cyclopentanol. Cells were Gram-reaction-negative, non-motile, irregular cocci that were 1.4\u20132.9 \u00b5m in diameter and occurred singly or in pairs. The strain grew at 20\u201342 \u00b0C (optimum 37 \u00b0C), at pH 6.0\u20137.75 (optimum pH 7.0\u20137.25) and in 0\u20130.35 M NaCl (optimum 0.1 M). The G+C content of the genomic DNA was 41.4 mol%. 16S rRNA gene sequencing revealed that the strain was a member of the genus\n                    <jats:italic>Methanosarcina<\/jats:italic>\n                    but that it clearly differed from all recognized species of this genus (93.1\u201397.9\u200a% sequence similarity). The phenotypic and phylogenetic features of strain HB-1\n                    <jats:sup>T<\/jats:sup>\n                    indicate that it represents a novel species of the genus\n                    <jats:italic>Methanosarcina<\/jats:italic>\n                    , for which the name\n                    <jats:italic>Methanosarcina horonobensis<\/jats:italic>\n                    sp. nov. is proposed. The type strain is HB-1\n                    <jats:sup>T<\/jats:sup>\n                    (\u200a=\u200aDSM 21571\n                    <jats:sup>T<\/jats:sup>\n                    \u200a=\u200aJCM 15518\n                    <jats:sup>T<\/jats:sup>\n                    \u200a=\u200aNBRC 102577\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijs.0.028548-0","type":"journal-article","created":{"date-parts":[[2010,11,26]],"date-time":"2010-11-26T22:26:37Z","timestamp":1290810397000},"page":"2503-2507","source":"Crossref","is-referenced-by-count":68,"title":["Methanosarcina horonobensis sp. nov., a methanogenic archaeon isolated from a deep subsurface Miocene formation"],"prefix":"10.1099","volume":"61","author":[{"given":"Satoru","family":"Shimizu","sequence":"first","affiliation":[{"name":"Horonobe Research Institute for the Subsurface Environment, Northern Advancement Center for Science and Technology, Horonobe-cho, Teshio-gun, Hokkaido 098-3221, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Upadhye","sequence":"additional","affiliation":[{"name":"Horonobe Research Institute for the Subsurface Environment, Northern Advancement Center for Science and Technology, Horonobe-cho, Teshio-gun, Hokkaido 098-3221, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoji","family":"Ishijima","sequence":"additional","affiliation":[{"name":"Horonobe Research Institute for the Subsurface Environment, Northern Advancement Center for Science and Technology, Horonobe-cho, Teshio-gun, Hokkaido 098-3221, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Naganuma","sequence":"additional","affiliation":[{"name":"Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","first-page":"521","article-title":"16S and 23S rRNA-like primers","volume-title":"Archaea: A Laboratory Manual: Methanogens","author":"Achenbach","year":"1995"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1139\/m86-025"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-37-2-169"},{"key":"r4","first-page":"55","article-title":"Hyperthermophilic and methanogenic archaea in oil fields","volume-title":"Petroleum Microbiology","author":"Christian","year":"2005"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.003772-0"},{"key":"r6","doi-asserted-by":"crossref","first-page":"3869","DOI":"10.1128\/AEM.64.10.3869-3877.1998","article-title":"Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation","volume":"64","author":"Dojka","year":"1998","journal-title":"Appl Environ 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